College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, P.R. China.
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, P.R. China.
Poult Sci. 2021 Feb;100(2):918-925. doi: 10.1016/j.psj.2020.11.029. Epub 2020 Nov 27.
Oxidative stress has always been a hot topic in poultry science. However, studies concerning the effects of redox status and glucose metabolism induced by hydrogen peroxide (HO) in the breast muscle of broilers have been rarely reported. This study was aimed to evaluate the impact of intraperitoneal injection of HO on oxidative damage and glycolysis metabolism of breast muscle in broilers. We also explored the activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway to provide possible mechanism of the redox imbalance. Briefly, a total of 320 one-day-old Arbor Acres chicks were randomly divided into 5 treatments with 8 replicates of 8 birds each (noninjected control, 0.75% saline-injected, 2.5, 5.0, and 10.0% HO-injected treatments). Saline group was intraperitoneally injected with physiological saline (0.75%) and HO groups received an intraperitoneal injection of HO The dosage of the injection was 1.0 mL/kg BW. All birds in the saline and HO groups were injected on days 16 and 37 of the experimental period. At 42 d of age, 40 birds (8 cages per group and one chicken per cage) were selected to be stunned electrically (50 V, alternating current, 400 Hz for 5 s each one), and then immediately slaughtered via exsanguination. The results showed that broilers in the HO injection group linearly exhibited higher contents of reactive oxygen species, carbonyl and malondialdehyde, and lower total antioxidant capacity and glutathione peroxidase activities. With the content of HO increased, the HO groups linearly downregulated the mRNA expressions of GPX, CAT, HMOX1, NQO1, and Nrf2 and its downstream target genes. In addition, HO increased serum activities of creatine kinase and lactate dehydrogenase. Meanwhile, in the pectoral muscle, the glycogen content was linearly decreased, and the lactate content was linearly increased in muscle of broilers injected with HO. In addition, the activities of glycolytic enzymes including pyruvate kinase, hexokinase, and lactate dehydrogenase were linearly increased after exposure to HO. In conclusion, HO injection could impair antioxidant status and enhance anaerobic metabolism of breast muscle in broilers.
氧化应激一直是家禽科学的热门话题。然而，关于过氧化氢（HO）对肉鸡胸肌中氧化还原状态和葡萄糖代谢影响的研究却很少有报道。本研究旨在评估腹腔注射 HO 对肉鸡胸肌氧化损伤和糖酵解代谢的影响。我们还探讨了核因子红细胞 2 相关因子 2（Nrf2）信号通路的激活，为氧化还原失衡的可能机制提供了依据。简而言之，将 320 只 1 日龄的爱拔益加小鸡随机分为 5 个处理组，每个处理组 8 个重复，每个重复 8 只鸡（未注射对照组、0.75%生理盐水注射组、2.5%、5.0%和 10.0%HO 注射组）。生理盐水组腹腔注射生理盐水（0.75%），HO 组腹腔注射 HO，注射剂量为 1.0mL/kgBW。生理盐水和 HO 组所有鸡均于实验期第 16 天和第 37 天注射。在 42 日龄时，选择 40 只鸡（每组 8 个笼，每个笼 1 只鸡）进行电击晕（50V，交流电，400Hz，每只鸡 5s），然后立即通过放血屠宰。结果表明，HO 注射组肉鸡的活性氧、羰基和丙二醛含量呈线性升高，总抗氧化能力和谷胱甘肽过氧化物酶活性呈线性降低。随着 HO 含量的增加，HO 组 GPX、CAT、HMOX1、NQO1 和 Nrf2 及其下游靶基因的 mRNA 表达呈线性下调。此外，HO 增加了血清肌酸激酶和乳酸脱氢酶的活性。同时，在胸肌中，HO 注射肉鸡的肌糖原含量呈线性降低，肌乳酸含量呈线性升高。此外，HO 暴露后，糖酵解酶如丙酮酸激酶、己糖激酶和乳酸脱氢酶的活性呈线性增加。综上所述，HO 注射可损害肉鸡胸肌的抗氧化状态，增强其无氧代谢。
