Laboratory of Molecular Toxicology, Faculty of Nature and Life Sciences, University of Mohammed Seddik Benyahia-Jijel, Jijel, Algeria.
ENFOCHEM, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.
Braz J Microbiol. 2021 Jun;52(2):749-759. doi: 10.1007/s42770-021-00464-9. Epub 2021 Mar 25.
Diclofenac (DCF) belongs to the class of nonsteroidal anti-inflammatory drugs, which is one of the most consumed by population and detected in raw sewage. Several studies have reported variable removal rates by biodegradation of diclofenac in wastewater treatment plants (WWTPs). This study deals with the evaluation of the biodegradation of DCF by a bacterial consortium (obtained from pure cultures of Enterobacter hormaechei D15 and Enterobacter cloacea D16), which were isolated from household compost and Algerian WWTP, respectively, as sole carbon source and by co-metabolism, using glucose as carbon source. A 98% removal rate of DCF was observed when it is used as the sole carbon source, whilst only 44% of DCF was removed in co-metabolic conditions. Two metabolites were identified using ultra-high-performance liquid chromatography coupled to electrospray injection tandem mass spectrometry analysis (UHPLC-ESI-MS/MS); one of them was identified as 4'-hydroxy-DCF, and the second metabolite was suspected to be a nitro derivative of DCF, according to comparison with the literature. Biodegradation of DCF by this bacterial consortium generates relatively safe final by-products.
双氯芬酸（DCF）属于非甾体类抗炎药，是人群中消耗最多的药物之一，也在未经处理的污水中被检出。多项研究报道了在污水处理厂（WWTP）中双氯芬酸通过生物降解的可变去除率。本研究评估了从家庭堆肥和阿尔及利亚 WWTP 中分别分离出的纯培养 Enterobacter hormaechei D15 和 Enterobacter cloacea D16 的细菌混合物作为唯一碳源和共代谢物对 DCF 的生物降解作用，使用葡萄糖作为碳源。当 DCF 作为唯一碳源时，观察到 98%的去除率，而在共代谢条件下仅去除了 44%的 DCF。使用超高效液相色谱-电喷雾串联质谱联用分析（UHPLC-ESI-MS/MS）鉴定出两种代谢物；其中一种被鉴定为 4'-羟基-DCF，第二种代谢物据文献比较推测为 DCF 的硝基衍生物。该细菌混合物对 DCF 的生物降解生成相对安全的最终副产物。
