Department of Urology, The Second Hospital, University of South China, Hengyang, 421001, Hunan, China.
College of Health Science and Nursing, Wuhan Polytechnic University, Wuhan, 420000, China.
BMC Cancer. 2021 Mar 25;21(1):316. doi: 10.1186/s12885-021-08034-w.
Wilms tumor (WT) is the most common malignant renal tumor in children. The aim of this study was to identify potential susceptibility gene of WT for better prognosis.
Weighted gene coexpression network analysis is used for the detection of clinically important biomarkers associated with WT.
In the study, 59 tissue samples from National Cancer Institute were pretreated for constructing gene co-expression network, while 224 samples also downloaded from National Cancer Institute were used for hub gene validation and module preservation analysis. Three modules were found to be highly correlated with WT, and 44 top hub genes were identified in these key modules eventually. In addition, both the module preservation analysis and gene validation showed ideal results based on other dataset with 224 samples. Meanwhile, Functional enrichment analysis showed that genes in module were enriched to sister chromatid cohesion, cell cycle, oocyte meiosis.
In summary, we established a gene co-expression network to identify 44 hub genes are closely to recurrence and staging of WT, and 6 of these hub genes was closely related to the poor prognosis of patients. Our findings revealed that those hub genes may be used as potential susceptibility gene for clinical diagnosis and prognosis of this tumor.
肾母细胞瘤（WT）是儿童中最常见的恶性肾肿瘤。本研究旨在鉴定 WT 相关的潜在易感性基因，以改善预后。
采用加权基因共表达网络分析方法检测与 WT 相关的临床重要生物标志物。
本研究中，59 份来自国家癌症研究所的组织样本被预处理用于构建基因共表达网络，同时还从国家癌症研究所下载了 224 份样本用于枢纽基因验证和模块保存分析。发现三个模块与 WT 高度相关，最终确定了 44 个关键模块中的顶级枢纽基因。此外，基于包含 224 个样本的其他数据集，模块保存分析和基因验证均显示出理想的结果。同时，功能富集分析表明模块中的基因富集到姐妹染色单体凝聚、细胞周期、卵母细胞减数分裂。
综上所述，我们建立了一个基因共表达网络，鉴定出 44 个与 WT 复发和分期密切相关的枢纽基因，其中 6 个枢纽基因与患者的不良预后密切相关。我们的研究结果表明，这些枢纽基因可能作为该肿瘤临床诊断和预后的潜在易感性基因。
