Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China.
Institute of Hygiene, Hangzhou Medical College, Hangzhou, Zhejiang 310013, China.
J Glob Antimicrob Resist. 2021 Jun;25:110-113. doi: 10.1016/j.jgar.2021.03.008. Epub 2021 Mar 22.
Achromobacter xylosoxidans is widely distributed in the environment and is increasingly recognised as a significant nosocomial pathogen in healthcare settings. However, the genomic and phylogenetic characteristics of this pathogen are not fully understood. Here we report the first genome sequence of a multidrug-resistant A. xylosoxidans ST273 strain (MTYH1) isolated from a wound infection in China.
Whole-genome sequencing of the strain was performed using an Illumina NovaSeq 6000 platform. Generated short reads were de novo assembled into contigs using SPAdes v.3.13.0. Antimicrobial resistance genes (ARGs) were identified using ResFinder 4.0. Phylogenetic analysis was conducted using NJ/UPGMA phylogeny (MAFFT v.7) based on core genome single nucleotide polymorphism data.
The draft genome sequence of A. xylosoxidans ST273 strain MTYH1 consists of 45 contigs comprising 6 643 045 bp with a GC content of 67.5%. According to the Achromobacter spp. multilocus sequence typing (MLST) scheme, MTYH1 belongs to ST273. MTYH1 is resistant to aminoglycosides, ciprofloxacin, aztreonam and cephalosporins. Five ARGs belonging to three different classes were identified in the genome, namely aac(6')-IIa, ant(2'')-Ia, bla, bla and sul1. Phylogenetic analysis showed that MTYH1 was not epidemiologically related to any of the strains retrieved from the NCBI GenBank database.
We unravelled the genome sequence of multidrug-resistant A. xylosoxidans ST273 clinical strain MTYH1 recovered from a wound infection in China. Our data may help to understand the antimicrobial resistance mechanisms, genomic features and phylogenetic characteristic of this bacterial pathogen.
阿克曼菌广泛分布于环境中，并且越来越被认为是医疗保健环境中一种重要的医院获得性病原体。然而，该病原体的基因组和系统发育特征尚未完全了解。本研究报告了一株从中国伤口感染中分离的多药耐药阿克曼菌 ST273 菌株（MTYH1）的全基因组序列。
使用 Illumina NovaSeq 6000 平台对该菌株进行全基因组测序。使用 SPAdes v.3.13.0 将生成的短读序列从头组装成 contigs。使用 ResFinder 4.0 识别抗生素耐药基因（ARGs）。使用基于核心基因组单核苷酸多态性数据的 NJ/UPGMA 系统发育（MAFFT v.7）进行系统发育分析。
阿克曼菌 ST273 菌株 MTYH1 的基因组序列草图由 45 个 contigs 组成，包含 6 643 045 bp，GC 含量为 67.5%。根据阿克曼菌属多位点序列分型（MLST）方案，MTYH1 属于 ST273。MTYH1 对氨基糖苷类、环丙沙星、氨曲南和头孢菌素类药物耐药。在基因组中鉴定出 5 个属于 3 个不同类别的 ARGs，分别为 aac(6')-IIa、ant(2'')-Ia、bla、bla 和 sul1。系统发育分析表明，MTYH1 与从 NCBI GenBank 数据库中检索到的任何菌株在流行病学上均无关联。
我们揭示了从中国伤口感染中分离的多药耐药阿克曼菌 ST273 临床菌株 MTYH1 的基因组序列。我们的数据可能有助于了解该细菌病原体的抗生素耐药机制、基因组特征和系统发育特征。
