泰国一所教学医院中多重耐药的木糖氧化无色杆菌携带抗生素耐药整合子 In687 的新型基因组岛 AcGI1。
AcGI1, a novel genomic island carrying antibiotic resistance integron In687 in multidrug resistant Achromobacter xylosoxidans in a teaching hospital in Thailand.
机构信息
Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bang Phli, Samut Prakan 10540, Thailand.
Institute of Infection and Global Health, University of Liverpool, Liverpool L69 3BE, UK.
出版信息
FEMS Microbiol Lett. 2020 Jul 1;367(14). doi: 10.1093/femsle/fnaa109.
This study investigated the genetic basis of multidrug resistance in two strains of Achromobacter xylosoxidans isolated from patients attending a hospital in Thailand in 2012. These isolates were highly resistant to cephalosporins, aminoglycosides, fluoroquinolones, co-trimoxazole and carbapenems. Whole genome sequencing revealed that the two isolates were not clonally related and identified a carbapenem resistance gene-habouring integron (In687), residing in a novel genomic island, AcGI1. This In687 shares 100% identical nucleotide sequence with ones found in Acinetobacter baumannii Aci 16, isolated from the same hospital in 2007. We report the first analysis of multidrug-resistant A. xylosoxidans isolated in Thailand, and the first example of this island in A. xylosoxidans. Our data support the idea that resistance has spread in Thailand via horizontal gene transfer between species and suggest the possibility of A. xylosoxidans may serve as a reservoir of antibiotic resistance, especially in hospital setting.
本研究调查了 2012 年从泰国一家医院就诊的患者中分离出的两株木糖氧化无色杆菌对多种药物耐药的遗传基础。这些分离株对头孢菌素、氨基糖苷类、氟喹诺酮类、复方新诺明和碳青霉烯类高度耐药。全基因组测序表明，这两种分离株没有克隆相关，并鉴定出一个携带碳青霉烯耐药基因的整合子（In687），位于一个新的基因组岛上，AcGI1。该 In687 与 2007 年从同一医院分离出的鲍曼不动杆菌 Aci 16 中发现的 In687 具有 100%相同的核苷酸序列。我们报告了在泰国首次分离出的多药耐药性木糖氧化无色杆菌的分析结果，也是该岛首次在木糖氧化无色杆菌中发现。我们的数据支持这样一种观点，即耐药性通过种间水平基因转移在泰国传播，并表明木糖氧化无色杆菌可能成为抗生素耐药性的储存库，尤其是在医院环境中。
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