Forensic Science Laboratory, Directorate of Forensic Science, General Directorate of Criminal Investigation and Forensic Science, Ministry of Interior, Manama, Kingdom of Bahrain.
Department of Molecular Medicine, College of Medical and Medicine Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain.
Sci Rep. 2021 Mar 25;11(1):6843. doi: 10.1038/s41598-021-86386-w.
This paper evaluates the forensic utility of 30 insertion-deletion polymorphism (indel) markers in a sample from the Bahraini population using the Qiagen Investigator DIPplex Kit. Allele frequencies and forensic stats of the 30 indels were investigated in 293 unrelated individuals from different governorates of the Kingdom of Bahrain. None of the markers showed significant deviation from Hardy Weinberg equilibrium except for HLD88 locus and no linkage disequilibrium were detected between all possible pair of the indel loci, assuming that these markers are independent and their allele frequencies can be used to calculate the match probabilities in the Bahraini population. The high power of discrimination (CPD = 0.9999999999998110) and the low combined match probability (CPM = 1.89 × 10) indicate that these markers are informative and can be successfully used for human identification in terms of forensics and paternity. Genetic distances and relatedness were displayed through multidimensional plotting and phylogenetic tree using various populations in the region. Our study showed that the Bahraini population was clustered with neighboring countries such as Kuwait and Emirates which indicates that these closely geographical regions share similar allele frequencies and are more genetically related than other reference population studied.
本文使用 Qiagen Investigator DIPplex 试剂盒评估了 30 个插入-缺失多态性（indel）标记在巴林人群样本中的法医学效用。在来自巴林王国不同省份的 293 名无关个体中，研究了 30 个 indel 的等位基因频率和法医学统计数据。除了 HLD88 基因座外，没有一个标记显示出与 Hardy-Weinberg 平衡有显著偏差，并且在所有可能的 indel 基因座对之间都没有检测到连锁不平衡，假设这些标记是独立的，它们的等位基因频率可以用于计算巴林人群中的匹配概率。高鉴别力（CPD=0.9999999999998110）和低联合匹配概率（CPM=1.89×10）表明，这些标记具有信息性，可以成功用于法医学和亲子鉴定中的个体识别。通过多维绘图和使用该地区的各种人群构建的系统发育树，显示了遗传距离和相关性。我们的研究表明，巴林人群与科威特和阿联酋等邻国聚类在一起，这表明这些地理位置相近的地区具有相似的等位基因频率，并且比其他研究的参考人群具有更强的遗传关系。
