Sun Shijing, Jiang Hui, Li Qiaoling, Liu Yufu, Gao Qiang, Liu Wei, Qin Yuming, Feng Yu, Peng Xiaowei, Xu Guanlong, Shen Qingchun, Fan Xuezheng, Ding Jiabo, Zhu Liangquan
National/OIE Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control (IVDC), Beijing, China.
Academy of Agriculture and Animal Husbandry Sciences, Hohhot, China.
Front Vet Sci. 2021 Mar 9;8:641022. doi: 10.3389/fvets.2021.641022. eCollection 2021.
Brucellosis, caused by spp., is an important zoonotic disease leading to enormous economic losses in livestock, posing a great threat to public health worldwide. The live attenuated strain S2, a safe and effective vaccine, is widely used in animals in China. However, S2 vaccination in animals may raise debates and concerns in terms of safety to primates, particularly humans. In this study, we used monkey as an animal model to evaluate the safety of the S2 vaccine strain on primates. In addition, we performed transcriptome analysis to determine gene expression profiling on monkeys immunized with the S2 vaccine. Our results suggested that the S2 vaccine was safe for monkeys. The transcriptome analysis identified 663 differentially expressed genes (DEGs), of which 348 were significantly upregulated and 315 were remarkably downregulated. The Gene Ontology (GO) classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these DEGs were involved in various biological processes (BPs), including the chemokine signaling pathway, actin cytoskeleton regulation, the defense response, immune system processing, and the type-I interferon signaling pathway. The molecular functions of the DEGs were mainly comprised of 2'-5'-oligoadenylate synthetase activity, double-stranded RNA binding, and actin-binding. Moreover, the cellular components of these DEGs included integrin complex, myosin II complex, and blood microparticle. Our findings alleviate the concerns over the safety of the S2 vaccine on primates and provide a genetic basis for the response from a mammalian host following vaccination with the S2 vaccine.
布鲁氏菌病由布鲁氏菌属细菌引起，是一种重要的人畜共患病，给家畜造成巨大经济损失，对全球公共卫生构成重大威胁。减毒活疫苗株S2是一种安全有效的疫苗，在中国被广泛用于动物。然而，动物接种S2疫苗可能会引发关于对灵长类动物尤其是人类安全性的争论和担忧。在本研究中，我们以猕猴作为动物模型来评估S2疫苗株对灵长类动物的安全性。此外，我们进行了转录组分析，以确定接种S2疫苗的猕猴的基因表达谱。我们的结果表明，S2疫苗对猕猴是安全的。转录组分析鉴定出663个差异表达基因（DEG），其中348个显著上调，315个显著下调。基因本体（GO）分类和京都基因与基因组百科全书（KEGG）通路分析表明，这些DEG参与了各种生物学过程（BP），包括趋化因子信号通路、肌动蛋白细胞骨架调节、防御反应、免疫系统过程和I型干扰素信号通路。DEG的分子功能主要包括2'-5'-寡腺苷酸合成酶活性、双链RNA结合和肌动蛋白结合。此外，这些DEG的细胞成分包括整合素复合体、肌球蛋白II复合体和血液微粒。我们的研究结果减轻了对S2疫苗对灵长类动物安全性的担忧，并为哺乳动物宿主接种S2疫苗后的反应提供了遗传基础。
