Möschl P, Keiler A, Szalay S, Mayr W R, Salem G, Glöckler M, Lubec G
Z Exp Chir. 1977;10(5):269-74.
A method appropriate for identification and quantification of circulating antibodies reacting with donorlungantigens is presented in allogenous lungtransplantation. Following incubation of native tissue-slides taken from the second removed but not grafted donor lung with the recipient's serum, the specific humoral antibodies are detected by indirect immunfluorescence. Quantifying evaluation is performed by serum-dilutions. The preexisting specific antibody-titer found in DLA-sensitized animals shows a significant correlation with the duration of graft function when compared with lymphocytotoxic antibody-titer. In unsensitized dogs a regular appearance of circulating specific antibodies can be seen beginning from the fourth or fifth postoperative day. The increasing antibody-titer during further course is a feature of a humoral graft rejection represented in the periphereal blood-circulation.
本文介绍了一种适用于同种异体肺移植中鉴定和定量与供体肺抗原发生反应的循环抗体的方法。将取自第二个切除但未移植的供体肺的天然组织切片与受体血清孵育后，通过间接免疫荧光检测特异性体液抗体。通过血清稀释进行定量评估。与淋巴细胞毒性抗体滴度相比，在DLA致敏动物中发现的预先存在的特异性抗体滴度与移植物功能持续时间显著相关。在未致敏的犬中，从术后第四或第五天开始可观察到循环特异性抗体的规律出现。在后续过程中抗体滴度的增加是外周血液循环中体液性移植排斥反应的一个特征。
