Jo Hyun, Kim Minsu, Cho Hyeontae, Ha Bo-Keun, Kang Sungtaeg, Song Jong Tae, Lee Jeong-Dong
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of Korea.
J Agric Food Chem. 2021 Apr 7;69(13):3836-3847. doi: 10.1021/acs.jafc.0c05830. Epub 2021 Mar 26.
This present study was to identify a novel candidate gene that contributes to the elevated α-linolenic acid (ALA, ω-3) concentration in PE2166 from mutagenesis of Pungsannamul. Major loci and were detected on chromosome 5 of soybean through quantitative trait loci mapping analyses of recombinant inbred lines. With next-generation sequencing of parental lines and Pungsannamul and recombinant analyses, a potential gene, (), controlling elevated ALA concentration was identified. is a homeodomain-like transcriptional regulator that may regulate the expression level of microsomal ω-3 fatty acid desaturase () genes responsible for the conversion of linoleic acid into ALA in the fatty acid biosynthetic pathway. In addition, we hypothesized that a combination of mutant alleles, , and either of microsomal delta-12 fatty acid desaturase 2-1 () could reduce the ω-6/ω-3 ratio. In populations where , , and genes were segregated, a combination of a allele from PE2166 and either of the variant alleles was sufficient to reduce the ω-6/ω-3 ratio in seeds.
本研究旨在通过丰山辣椒的诱变鉴定出一个导致PE2166中α-亚麻酸（ALA，ω-3）浓度升高的新候选基因。通过对重组自交系进行数量性状基因座定位分析，在大豆的5号染色体上检测到了主要基因座。通过对亲本系、丰山辣椒进行新一代测序和重组分析，鉴定出一个潜在的控制ALA浓度升高的基因（）。是一种类同源结构域转录调节因子，可能调节脂肪酸生物合成途径中负责将亚油酸转化为ALA的微粒体ω-3脂肪酸去饱和酶（）基因的表达水平。此外，我们推测突变等位基因、与微粒体δ-12脂肪酸去饱和酶2-1（）中的任何一个组合可以降低ω-6/ω-3比率。在、和基因分离的群体中，来自PE2166的等位基因与任何一个变异等位基因的组合足以降低种子中的ω-6/ω-3比率。
