Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China.
Shanghai Engineering Research Center of Biotransformation of Organic Solid Waste, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, PR China; Engineering Research Center for Nanophotonics and Advanced Instrument, Ministry of Education, East China Normal University, Shanghai 200062, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China.
Bioresour Technol. 2022 Jan;344(Pt B):126257. doi: 10.1016/j.biortech.2021.126257. Epub 2021 Nov 6.
This study revealed the effects and regulation mechanisms on antibiotic resistance genes (ARGs) dissemination during anaerobic co-digestion (AcoD) of food waste and sludge under the exposure of tetracycline, sulfamethoxazole (SMZ) and erythromycin (ERY). Results indicated antibiotics significantly increased the abundance of ARGs, and selectively enriched integron gene, suggesting antibiotics promoted the dissemination of ARGs. Procrustes analysis indicated that bacterial community, integrons and physicochemical properties displayed significant correlations with ARGs, and they respectively contributed 10.61%, 6.94% and 2.97% of explanations on ARGs variation. Especially, the maximum combined contribution (48.6%) of bacterial community and integrons, implying their significances on ARGs alteration. Metatranscriptomic analysis further demonstrated antibiotics upregulated the expressions of total ARGs and virulence factors, raising potential risks. The proposed mechanisms for ARGs dissemination facilitated by antibiotics might be attributed to the changes of ARGs-regulated functions for inducing DNA/cell damage and DNA conjugation during AcoD.
本研究揭示了在四环素、磺胺甲恶唑（SMZ）和红霉素（ERY）暴露下，食物垃圾和污泥厌氧共消化（AcoD）过程中抗生素耐药基因（ARGs）传播的影响和调控机制。结果表明，抗生素显著增加了 ARGs 的丰度，并选择性地富集了整合子基因，表明抗生素促进了 ARGs 的传播。普罗克汝斯分析表明，细菌群落、整合子和理化性质与 ARGs 呈现显著相关性，分别解释了 ARGs 变化的 10.61%、6.94%和 2.97%。特别是，细菌群落和整合子的最大综合贡献（48.6%），表明它们对 ARGs 变化的重要性。宏转录组学分析进一步表明，抗生素上调了总 ARGs 和毒力因子的表达，增加了潜在风险。抗生素促进 ARGs 传播的机制可能归因于 ARGs 调节功能的变化，这些功能在 AcoD 过程中诱导了 DNA/细胞损伤和 DNA 接合。
