Laboratorio de Biomedicina Molecular 2. Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Ciudad de México, México.
Deparmento de Infectómica y Patogénesis Molecular, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, México.
Exp Parasitol. 2021 May;224:108103. doi: 10.1016/j.exppara.2021.108103. Epub 2021 Mar 24.
In this work the effect of (-)-epicatechin on the development of amebic liver abscess in hamsters was evaluated. (-)-epicatechin is a flavonoid present in plants that possesses various biological properties, including its activity against some protozoal parasites; however its antiamebic activity in a living model had not been evaluated. Syrian golden hamsters were intrahepatically inoculated with 1x10E. histolytica trophozoites, three days after inoculation they received nine intraperitoneal doses of (-)-epicatechin (10 mg/100 g) every 48 h. Animals without treatments and treated with metronidazole were included as controls. Macroscopic characteristics of the hepatic abscess, histopathological analysis of the tissue and the levels of inflammatory cytokines were determined. (-)-epicatechin produced a decrease in liver abscess progression being observed only 9.49% of damage compared to 84% shown by untreated animals. During treatment with (-)-epicatechin hepatic tissue showed signs of liver repair and absence of amoebae. Additionally, (-)-epicatechin produced a modulating effect on inflammatory cytokines TNF-α, IL-1β and IL-10. All these events observed in animals treated with (-)-epicatechin could contribute to the elimination of trophozoites and liver healing.
在这项工作中，评估了 (-)-表儿茶素对仓鼠阿米巴肝脓肿发展的影响。(-)-表儿茶素是一种存在于植物中的类黄酮，具有多种生物学特性，包括对一些原生动物寄生虫的活性；然而，其在活体模型中的抗阿米巴活性尚未得到评估。叙利亚金仓鼠肝内接种 1x10E。组织内滋养体，接种后 3 天，每 48 小时接受 9 次腹腔内 (-)-表儿茶素（10 mg/100 g）剂量。未治疗和用甲硝唑治疗的动物被包括作为对照。测定肝脓肿的宏观特征、组织的组织病理学分析和炎症细胞因子的水平。(-)-表儿茶素可减少肝脓肿的进展，与未治疗动物的 84%相比，仅观察到 9.49%的损伤。在 (-)-表儿茶素治疗期间，肝组织显示出肝脏修复的迹象，并且没有变形虫。此外，(-)-表儿茶素对 TNF-α、IL-1β 和 IL-10 等炎症细胞因子产生了调节作用。在 (-)-表儿茶素治疗的动物中观察到的所有这些事件都可能有助于消除滋养体和肝脏愈合。
