Dorozhko Elena V, Gashevskay Anna S, Korotkova Elena I, Barek Jiri, Vyskocil Vlastimil, Eremin Sergei A, Galunin Evgeny V, Saqib Muhammad
School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia.
School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia; Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 12843, Prague 2, Czech Republic.
Talanta. 2021 Jun 1;228:122174. doi: 10.1016/j.talanta.2021.122174. Epub 2021 Feb 4.
A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive determination of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of the GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 μg·kg, with a limit of detection 0.08 μg·kg. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks, and soil samples.
在本研究中，首次合成了一种带有铜纳米颗粒的半抗原-蛋白质缀合物（Hap-Car-BSA@CuNPs）用于西维因的测定。该缀合物中的铜纳米颗粒（CuNPs）在直接固相竞争法测定不同作物面粉中西维因残留量时用作电化学标记物。通过在金-石墨电极（GGE）上对铜进行线性扫描阳极溶出伏安法（LSASV）（通过累积的元素铜的电化学溶出）来读取信号。为了在GGE表面形成针对西维因的单克隆抗体识别受体层，使用1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐（EDC）和1-羟基-2,5-吡咯烷二酮（NHS）作为最佳的共价交联剂。通过电化学免疫传感器对西维因检测时，优化了抗体和Hap-Car-BSA@CuNPs缀合物的浓度。该电化学免疫传感器可用于高灵敏度测定面粉样品中西维因残留量，浓度范围为0.8 - 32.3 μg·kg，检测限为0.08 μg·kg。本研究为监测其他食品、饮料和土壤样品中西维因的新方法铺平了道路。
