Behera Kartik, Mutharani Bhuvanenthiran, Chang Yen-Hsiang, Kumari Monika, Chiu Fang-Chyou
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan 333, Taiwan.
Department of General Dentistry, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.
Polymers (Basel). 2024 Jan 4;16(1):162. doi: 10.3390/polym16010162.
Dimetridazole (DMZ) is a derivative of nitroimidazole and is a veterinary drug used as an antibiotic to treat bacterial or protozoal infections in poultry. The residues of DMZ cause harmful side effects in human beings. Thus, we have constructed a superior electrocatalyst for DMZ detection. A copper (Cu)-integrated poly(aniline) (PANI) electrocatalyst (PANI-Cu@BSA) was prepared by using a one-step method of biomimetic mineralization and polymerization using bovine serum albumin (BSA) as a stabilizer. Then, the synthesized PANI-Cu@BSA was encapsulated with reduced graphene oxide (rGO) using an ultrasonication method. The PANI-Cu@BSA/rGO nanocomposite had superior water dispersibility, high electrical conductivity, and nanoscale particles. Moreover, a PANI-Cu@BSA/rGO nanocomposite-modified, screen-printed carbon electrode was used for the sensitive electrochemical detection of DMZ. In phosphate buffer solution, the PANI-Cu@BSA/rGO/SPCE displayed a current intensity greater than PANI-Cu@BSA/SPCE, rGO/SPCE, and bare SPCE. This is because PANI-Cu@BSA combined with rGO increases fast electron transfer between the electrode and analyte, and this synergy results in analyte-electrode junctions with extraordinary conductivity and active surface areas. PANI-Cu@BSA/rGO/SPCE had a low detection limit, a high sensitivity, and a linear range of 1.78 nM, 5.96 μA μM cm, and 0.79 to 2057 μM, respectively. The selective examination of DMZ was achieved with interfering molecules, and the PANI-Cu@BSA/rGO/SPCE showed excellent selectivity, stability, repeatability, and practicability.
二甲硝唑（DMZ）是一种硝基咪唑衍生物，是一种用于治疗家禽细菌或原生动物感染的兽药抗生素。DMZ的残留会对人类产生有害副作用。因此，我们构建了一种用于检测DMZ的优质电催化剂。通过以牛血清白蛋白（BSA）为稳定剂，采用仿生矿化和聚合一步法制备了一种铜（Cu）集成聚苯胺（PANI）电催化剂（PANI-Cu@BSA）。然后，使用超声方法用还原氧化石墨烯（rGO）包裹合成的PANI-Cu@BSA。PANI-Cu@BSA/rGO纳米复合材料具有优异的水分散性、高导电性和纳米级颗粒。此外，将PANI-Cu@BSA/rGO纳米复合材料修饰的丝网印刷碳电极用于DMZ的灵敏电化学检测。在磷酸盐缓冲溶液中，PANI-Cu@BSA/rGO/SPCE显示出比PANI-Cu@BSA/SPCE、rGO/SPCE和裸SPCE更高的电流强度。这是因为PANI-Cu@BSA与rGO结合增加了电极与分析物之间的快速电子转移，这种协同作用导致分析物-电极结具有非凡的导电性和活性表面积。PANI-Cu@BSA/rGO/SPCE的检测限低、灵敏度高，线性范围分别为1.78 nM、5.96 μA μM cm和0.79至2057 μM。通过干扰分子实现了对DMZ的选择性检测，PANI-Cu@BSA/rGO/SPCE表现出优异 的选择性、稳定性、重复性和实用性。
