Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil.
Programa de Pós-graduação Multicêntrico em Ciências Fisiológicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil; Faculdade de Medicina, UFVJM, Diamantina, Brazil.
Life Sci. 2021 Jun 15;275:119411. doi: 10.1016/j.lfs.2021.119411. Epub 2021 Mar 24.
Menopause is a female condition induced by a reduction of ovarian hormone and is related to an increase in cardiovascular diseases in women. We have shown that severe calorie restriction (SCR) from birth reduces the cardiometabolic risk in adult male Wistar rats. In this study, we investigated the effects of SCR from birth to adulthood on cardiovascular function of ovariectomized rats.
From birth to adulthood, rats were daily fed ad libitum (control group - C) or with 50% of the amount consumed by the control group (calorie-restricted group - R). At 90 days, half of the rats in each group underwent bilateral ovariectomy (OVX), totaling 4 groups: C-Sham, C-OVX, R-Sham, R-OVX. Systolic blood pressure (SBP), heart rate (HR) and, double product (DP) index were recorded by tail-cuff plethysmography. Cardiac function was analyzed by the Langendorff technique and cardiomyocyte diameter was accessed by histologic analysis. Additionally, cardiac SERCA2 content and redox status were evaluated.
C-OVX rats exhibited reduced cardiac function and cardiac non-enzymatic total antioxidant capacity (TAC). R-Sham animals showed reduced SBP, DP, HR, improved cardiac function, reduced cardiac protein carbonyl derivatives and increased TAC, catalase, and superoxide dismutase activities. R-OVX rats maintained reduced SBP, DP, HR, and increased contractility and relaxation indexes. R-Sham and R-OVX rats exhibited preserved heart mass and reduced cardiomyocyte diameter. Cardiac SERCA2 content did not differ between the groups.
Taken together, our findings show cardioprotective effects of SCR from birth in adult ovariectomized rats.
绝经是由卵巢激素减少引起的女性特有的状况，与女性心血管疾病的增加有关。我们已经表明，从出生开始进行严格的热量限制（SCR）会降低成年雄性 Wistar 大鼠的心脏代谢风险。在这项研究中，我们研究了从出生到成年期的 SCR 对去卵巢大鼠心血管功能的影响。
从出生到成年，大鼠每天自由进食（对照组-C）或摄入对照组 50%的食量（热量限制组-R）。在 90 天，每组一半的大鼠接受双侧卵巢切除术（OVX），总共 4 组：C-Sham、C-OVX、R-Sham、R-OVX。通过尾套容积描记法记录收缩压（SBP）、心率（HR）和双乘积（DP）指数。通过 Langendorff 技术分析心脏功能，通过组织学分析评估心肌细胞直径。此外，还评估了心脏 SERCA2 含量和氧化还原状态。
C-OVX 大鼠表现出心脏功能降低和心脏非酶总抗氧化能力（TAC）降低。R-Sham 动物表现出 SBP、DP、HR 降低，心脏功能改善，心脏蛋白羰基衍生物减少，TAC、过氧化氢酶和超氧化物歧化酶活性增加。R-OVX 大鼠保持 SBP、DP、HR 降低，收缩和舒张指数增加。R-Sham 和 R-OVX 大鼠表现出心脏质量保持和心肌细胞直径减小。心脏 SERCA2 含量在各组之间没有差异。
综上所述，我们的研究结果表明，从出生开始的 SCR 对成年去卵巢大鼠具有心脏保护作用。
