Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, Iran.
Life Sci. 2022 Apr 1;294:120367. doi: 10.1016/j.lfs.2022.120367. Epub 2022 Jan 29.
Estrogen has an anti-obesity effect and plays an important role in improving cardiometabolic disorders. Weight loss and reduction in calorie intake impede the development of obesity-related cardiometabolic risk factors. Therefore, we investigated the substitution of calorie restriction for effects of estrogen on cardiometabolic risk factors and oxidative stress in obese postmenopausal rat model. In this study, adult female Wistar rats were allocated into Sham and ovariectomized (OVX) groups and were given standard diet (SD) or 60% high-fat diet (HFD) or 30% calorie restriction (CR) for 16 weeks, following this, animals received E2 (17-β estradiol; 1 mg/kg; i.p.) every four days for 4 weeks. Results showed that HFD elevated the body weight, BMI, food intake, and blood glucose (BG) level in both sham and OVX groups. In addition, HFD had negative effects on lipid profile and oxidative stress in these groups. Whereas CR decreased these indices in both Sham and OVX groups fed an HFD, it could not diminish the BG level in the OVX-HFD group. E2 treatment in OVX animals with or without CR reduced body weight, BMI, food intake, and BG level, and also had positive effects on lipid profile alterations and oxidative stress reduction. In comparison, no significant differences were observed regarding the effects of E2 with CR between two groups for body weight, lipid profile, BG, and oxidative stress in the OVX-HFD rats. Overall, CR prevents and ameliorates cardiometabolic risk factors induced by obesity in postmenopausal conditions and is also a good candidate for E2 substitution.
雌激素具有抗肥胖作用，在改善心血管代谢紊乱方面发挥着重要作用。减轻体重和减少热量摄入会阻碍与肥胖相关的心血管代谢危险因素的发展。因此，我们研究了用热量限制替代雌激素对肥胖绝经后大鼠模型中心血管代谢危险因素和氧化应激的影响。在这项研究中，成年雌性 Wistar 大鼠被分为假手术（Sham）和卵巢切除（OVX）组，并给予标准饮食（SD）或 60%高脂肪饮食（HFD）或 30%热量限制（CR）16 周，随后，动物接受 17-β雌二醇（E2；1mg/kg；腹腔注射）每四天一次，共 4 周。结果表明，HFD 增加了 Sham 和 OVX 两组的体重、BMI、食物摄入量和血糖（BG）水平。此外，HFD 对这些组的血脂谱和氧化应激产生了负面影响。而 CR 降低了这两组中 HFD 喂养的这些指标，但不能降低 OVX-HFD 组的 BG 水平。E2 治疗 OVX 动物，无论是否进行 CR，均降低了体重、BMI、食物摄入量和 BG 水平，并且对血脂谱的改变和氧化应激的减轻也有积极作用。相比之下，在 OVX-HFD 大鼠中，E2 与 CR 联合应用对体重、血脂谱、BG 和氧化应激的影响在两组之间没有显著差异。总的来说，CR 可预防和改善绝经后肥胖引起的心血管代谢危险因素，也是替代 E2 的良好候选物。
