Das Sambit K, Kumar Sanath H, Nayak Binaya B, Lekshmi Manjusha
Quality Control Laboratory, Post-Harvest Technology, ICAR- Central Institute of Fisheries Education, Versova, Mumbai, 400061, India.
Curr Microbiol. 2021 May;78(5):1973-1980. doi: 10.1007/s00284-021-02447-3. Epub 2021 Mar 27.
In this study, we investigated the incidence of Cronobacter spp. in seafood collected from retail fish markets of Mumbai, India. A total of 50 samples comprising fresh finfish (n = 32), shellfish (n = 6), dried fish (n = 9) and water (n = 3) were analyzed for Cronobacter spp. by selective enrichment, isolation and biochemical tests. Of 145 isolates presumptively identified as Cronobacter spp. by biochemical tests, 37 were confirmed as Cronobacter spp. by Polymerase Chain Reaction (PCR) specific to the internal transcribed spacer (ITS) regions. Based on the partial ITS gene sequence analysis, 35 isolates were identified as Cronobacter malonaticus and two as Cronobacter sakazakii. The highest incidence of Cronobacter spp. was in dried fish (55.6%), followed by shellfish (33.3%). The virulence gene ompA was detected in two Cronobacter sakazakii isolates. This is the first report of the incidence of Cronobacter spp. in fresh and dried seafood from India, which highlights the need to focus on this emerging pathogen in tropical seafood.
在本研究中，我们调查了印度孟买零售鱼市场采集的海鲜中阪崎肠杆菌属的发生率。共分析了50个样本，包括新鲜有鳍鱼类（n = 32）、贝类（n = 6）、干鱼（n = 9）和水（n = 3）中的阪崎肠杆菌属，通过选择性富集、分离和生化试验进行检测。在通过生化试验初步鉴定为阪崎肠杆菌属的145株分离物中，37株通过针对内转录间隔区（ITS）区域的聚合酶链反应（PCR）确认为阪崎肠杆菌属。基于部分ITS基因序列分析，35株分离物被鉴定为阪崎肠杆菌，两株为阪崎肠杆菌。阪崎肠杆菌属的发生率最高的是干鱼（55.6%），其次是贝类（33.3%）。在两株阪崎肠杆菌分离物中检测到毒力基因ompA。这是关于印度新鲜和干海鲜中阪崎肠杆菌属发生率的首次报告，突出了在热带海鲜中关注这种新出现病原体的必要性。
