Budi Hendrik Setia, Pebriani Indah
Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Research Center, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
Contemp Clin Dent. 2020 Jul-Sep;11(3):245-248. doi: 10.4103/ccd.ccd_394_19. Epub 2020 Nov 26.
A new case of an allergic reaction due to the use of toothpaste was found in California, United States, causing the death of an 11-year-old girl. Ingredients contained in toothpaste suspected as a cause of allergies are milk protein compounds.
The aim is to compare the protein level and molecular weight in children's toothpaste.
Stratified random sampling.
Samples used were children's toothpaste products in society, namely, Pepsodent, Cussons, Enzyme, Kodomo, Formula, Colgate, the toothpaste contain recaldent of GC Tooth Moose and pure cow's milk. Those samples were divided into eight groups, randomly selected according to the purpose (stratified random sampling), and then coded to maintain product confidentiality. Meanwhile, samples used as comparison groups were recaldent paste and pure cow's milk.
Each sample was analyzed for protein content using a biuret test and protein molecular weight using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis test. The protein content in toothpaste is compared with the similarity of the molecular protein weight in toothpaste that contains recaldent. Protein was found in samples 2, 3, 5, 6, 7, and 8 through a biuret test, with a concentration of 1.82; 1.53; 2.76; 1.92; 1.85; and 3.2 μg/mL. However, the protein bands were only found in sample 5 with a molecular weight of 20.7 kDa, sample 6 with a molecular weight of 19.1 kDa, sample 7 with a molecular weight of 17.7 kDa, and sample 8 with 2 bands, namely, 28.7 and 39.7 kDa.
We found the presence of protein in children's toothpaste. The protein molecular weight of recaldent paste is 17.7 kDa. Toothpaste containing protein with its molecular weight similar to recaldent's are found in samples 5 and 6.
在美国加利福尼亚州发现了一例因使用牙膏引发过敏反应的新病例，导致一名11岁女孩死亡。疑似导致过敏的牙膏成分是牛奶蛋白化合物。
目的是比较儿童牙膏中的蛋白质水平和分子量。
分层随机抽样。
使用的样本是社会上的儿童牙膏产品，即佳洁士、强生、酵素、儿童牙膏、配方牙膏、高露洁、含有GC护齿健齿素的牙膏以及纯牛奶。这些样本被分为八组，根据目的进行随机选择（分层随机抽样），然后进行编码以保持产品的保密性。同时，用作对照组的样本是护齿健齿素牙膏和纯牛奶。
使用双缩脲试验分析每个样本的蛋白质含量，并使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳试验分析蛋白质分子量。将牙膏中的蛋白质含量与含有护齿健齿素的牙膏中蛋白质分子量的相似性进行比较。通过双缩脲试验在样本2、3、5、6、7和8中发现了蛋白质，浓度分别为1.82；1.53；2.76；1.92；1.85；和3.2μg/mL。然而，仅在样本5中发现了分子量为20.7 kDa的蛋白条带，样本6中为19.1 kDa，样本7中为17.7 kDa，样本8中有两条带，即28.7和39.7 kDa。
我们发现儿童牙膏中存在蛋白质。护齿健齿素牙膏的蛋白质分子量为17.7 kDa。在样本5和6中发现了含有与护齿健齿素分子量相似的蛋白质的牙膏。
