Yu Yutian, Dong Jie, Wang Yanlu, Gong Xi
Human Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, China.
PeerJ. 2021 Mar 17;9:e11081. doi: 10.7717/peerj.11081. eCollection 2021.
Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in . In recent years, the overuse of antibiotics has led to an increase in antimicrobial resistance, which greatly reduces the efficacy of antibacterial drugs and affects people's health. Therefore, several researchers are focused on finding other materials, which could replace or supplement antibiotic treatment.
was treated with water, acetone and essential oils, respectively. The antibacterial activity was assessed using the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), the dry weight and the wet weight of the cells. To explore the antibacterial mechanism of the oil, the RNA-Seq analysis was adopted under three different treatments. Finally, the expression of related genes was verified by Quantitative PCR.
In this study, we showed that the essential oil exerted a strong antibacterial effect. Our results showed that the inhibitory efficiency increased with increasing of the concentration of essential oil. RNA-seq analysis indicated that the essential oil inhibited the growth of by inhibiting the metabolism, chemotaxis, and adhesion, meanwhile, life activities were maintained by enhancing resistance reactions. These results are contributed to uncover the antimicrobial mechanisms of essential oils against , and the essential oil could be applied as an antibacterial agent to replace or ally with antibiotic.
转录组分析在阐明基因表达调控的复杂性方面起着核心作用。近年来，抗生素的过度使用导致抗菌耐药性增加，这大大降低了抗菌药物的疗效并影响人们的健康。因此，一些研究人员专注于寻找其他可替代或补充抗生素治疗的材料。
分别用水、丙酮和精油处理。使用最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、细胞干重和湿重评估抗菌活性。为了探索精油的抗菌机制，在三种不同处理下采用RNA测序分析。最后，通过定量PCR验证相关基因的表达。
在本研究中，我们表明该精油具有很强的抗菌作用。我们的结果表明，抑制效率随着精油浓度的增加而提高。RNA测序分析表明，该精油通过抑制代谢、趋化性和黏附来抑制其生长，同时通过增强抗性反应维持生命活动。这些结果有助于揭示精油对其的抗菌机制，并且该精油可作为抗菌剂应用以替代抗生素或与抗生素联合使用。
