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用于工程化生物合成的微生物可溶性芳香类异戊二烯基转移酶

Microbial soluble aromatic prenyltransferases for engineered biosynthesis.

作者信息

Chen He-Ping, Abe Ikuro

机构信息

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, Hubei, 430074, PR China.

出版信息

Synth Syst Biotechnol. 2021 Mar 15;6(2):51-62. doi: 10.1016/j.synbio.2021.02.001. eCollection 2021 Jun.

DOI:10.1016/j.synbio.2021.02.001
PMID:33778178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7973389/
Abstract

Prenyltransferase (PTase) enzymes play crucial roles in natural product biosynthesis by transferring isoprene unit(s) to target substrates, thereby generating prenylated compounds. The prenylation step leads to a diverse group of natural products with improved membrane affinity and enhanced bioactivity, as compared to the non-prenylated forms. The last two decades have witnessed increasing studies on the identification, characterization, enzyme engineering, and synthetic biology of microbial PTase family enzymes. We herein summarize several examples of microbial soluble aromatic PTases for chemoenzymatic syntheses of unnatural novel prenylated compounds.

摘要

异戊烯基转移酶（PTase）通过将异戊二烯单元转移到目标底物上，在天然产物生物合成中发挥关键作用，从而生成异戊烯基化化合物。与未异戊烯基化的形式相比，异戊烯基化步骤产生了一类具有更高膜亲和力和更强生物活性的天然产物。在过去二十年中，对微生物PTase家族酶的鉴定、表征、酶工程和合成生物学的研究不断增加。我们在此总结了几个用于化学酶法合成非天然新型异戊烯基化化合物的微生物可溶性芳香族PTase的例子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/17445c5dda0b/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/1fe14dd1ff96/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/c0942909a1ff/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/c7c490e7ab3f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/d260939c757e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/3d6b2f477dcc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/e824276ff42a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/3e3b8674440f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/73f381d6feb8/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/87672288febc/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/be48e5910bbd/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/153ee324f908/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/1fdbe6753558/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/17445c5dda0b/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/1fe14dd1ff96/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/c0942909a1ff/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/c7c490e7ab3f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/d260939c757e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/3d6b2f477dcc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/e824276ff42a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/3e3b8674440f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/73f381d6feb8/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/87672288febc/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/be48e5910bbd/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/153ee324f908/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/1fdbe6753558/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67c0/7973389/17445c5dda0b/gr13.jpg

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