Gu Wenjia, Dong Shi-Hui, Sarkar Snigdha, Nair Satish K, Schmidt Eric W
Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT, United States.
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Methods Enzymol. 2018;604:113-163. doi: 10.1016/bs.mie.2018.03.002. Epub 2018 May 4.
Cyanobactin biosynthetic enzymes have exceptional versatility in the synthesis of natural and unnatural products. Cyanobactins are ribosomally synthesized and posttranslationally modified peptides synthesized by multistep pathways involving a broad suite of enzymes, including heterocyclases/cyclodehydratases, macrocyclases, proteases, prenyltransferases, methyltransferases, and others. Here, we describe the enzymology and structural biology of cyanobactin biosynthetic enzymes, aiming at the twin goals of understanding biochemical mechanisms and biosynthetic plasticity. We highlight how this common suite of enzymes may be utilized to generate a large array or structurally and chemically diverse compounds.
蓝细菌素生物合成酶在天然和非天然产物的合成中具有非凡的通用性。蓝细菌素是通过多步途径进行核糖体合成和翻译后修饰的肽，这些途径涉及一系列广泛的酶，包括杂环化酶/环脱水酶、大环化酶、蛋白酶、异戊烯基转移酶、甲基转移酶等。在此，我们描述蓝细菌素生物合成酶的酶学和结构生物学，旨在实现理解生化机制和生物合成可塑性这两个目标。我们强调了如何利用这套常见的酶来生成大量结构和化学性质多样的化合物。
