Zhang Lei, Gleason Cynthia
Plant Pathology Department, Washington State University, Pullman, WA 9916.
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
J Nematol. 2021 Oct 12;53. doi: 10.21307/jofnem-2021-084. eCollection 2021.
is a root-knot nematode that is a major pest of potato in the northwestern United States. Due to the lack of resistance against root-knot nematodes in potato, research has been undertaken to understand the -potato interaction at the molecular level. To identify the nematode genes that are playing roles in parasitism, we have performed transcriptome analyses on pre-parasitic and parasitic juveniles in susceptible potato. We compared gene expression profiles and identified genes that were significantly up- or down-regulated during nematode parasitism. Because parasitism proteins are typically secreted by the nematode to facilitate infection of host roots, we focused on the genes that encoded proteins that were predicted to be secreted. We found that approximately 34% (43/127) of the genes in the predicted secretome encoded proteins with no significant homology in the public genome databases, and 12% (15/127) encoded either a known effector, putative effectors or putative esophageal gland cell proteins. The transcriptome analyses of at the pre-parasitic and parasitic life stages shed light on the genes involved in nematode parasitism.
是一种根结线虫，是美国西北部马铃薯的主要害虫。由于马铃薯对根结线虫缺乏抗性，因此开展了研究以在分子水平上了解[线虫名称]与马铃薯的相互作用。为了鉴定在寄生过程中起作用的线虫基因，我们对易感马铃薯中寄生前和寄生期的幼虫进行了转录组分析。我们比较了基因表达谱，并鉴定了在线虫寄生期间显著上调或下调的基因。由于寄生蛋白通常由线虫分泌以促进对宿主根的感染，我们重点关注编码预测会被分泌的蛋白质的基因。我们发现，预测分泌组中的基因约34%（43/127）编码的蛋白质在公共基因组数据库中无明显同源性，12%（15/127）编码已知效应子、假定效应子或假定食管腺细胞蛋白。对[线虫名称]寄生前和寄生生命阶段的转录组分析揭示了参与线虫寄生的基因。
