Anderson D, Goyle S, Phillips B J, Tee A, Beech L, Butler W H
British Industrial Biological Research Association, Carshalton, Surrey, UK.
Br J Ind Med. 1988 Apr;45(4):269-74. doi: 10.1136/oem.45.4.269.
Since the Bhopal disaster, in which the causal agent was methyl isocyanate (MIC), exposed people have complained of various disorders including neuromuscular dysfunction. In an attempt to gain some information about the response of muscle tissue to MIC its effects were investigated in cells in culture isolated from muscle of 2 day old rats. After treatment with a range of MIC concentrations (0.025-0.5 microliter/5 ml culture) the total number of nuclei of the two main cell types (fibroblasts and myoblasts) and the number of nuclei in muscle fibres (myotubes) were recorded. At lower doses which had little effect on the total number of nuclei, the formation of muscle fibres--that is, fusion of muscle cells--was prevented as the proportion of nuclei in myotubes was decreased. At higher doses both cell types were killed. This would suggest either an effect on muscle differentiation or a selective toxicity towards myoblasts. The observations were supported by light and electron microscopy.
自博帕尔灾难发生以来，其致病因子为异氰酸甲酯（MIC），接触者出现了包括神经肌肉功能障碍在内的各种病症。为了获取一些关于肌肉组织对MIC反应的信息，对从2日龄大鼠肌肉分离的培养细胞中MIC的作用进行了研究。用一系列MIC浓度（0.025 - 0.5微升/5毫升培养液）处理后，记录了两种主要细胞类型（成纤维细胞和成肌细胞）的细胞核总数以及肌纤维（肌管）中的细胞核数量。在对细胞核总数影响较小的较低剂量下，由于肌管中细胞核比例降低，肌肉纤维的形成即肌肉细胞的融合受到了阻碍。在较高剂量下，两种细胞类型均被杀死。这表明要么对肌肉分化有影响，要么对成肌细胞有选择性毒性。这些观察结果得到了光学显微镜和电子显微镜的支持。
