Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana 46202, United States.
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, United States.
J Am Soc Mass Spectrom. 2021 Jun 2;32(6):1312-1318. doi: 10.1021/jasms.0c00460. Epub 2021 Mar 29.
Top-down mass spectrometry (MS) investigates intact proteoforms for proteoform identification, characterization, and quantification. Data visualization plays an essential role in top-down MS data analysis because proteoform identification and characterization often involve manual data inspection to determine the molecular masses of highly charged ions and validate unexpected alterations in identified proteoforms. While many software tools have been developed for MS data visualization, there is still a lack of web-based visualization software designed for top-down MS. Here, we present TopMSV, a web-based tool for top-down MS data processing and visualization. TopMSV provides interactive views of top-down MS data using a web browser. It integrates software tools for spectral deconvolution and proteoform identification and uses analysis results of the tools to annotate top-down MS data.
自上而下的质谱（MS）分析完整的蛋白质形式，用于蛋白质形式的鉴定、表征和定量。数据可视化在自上而下的 MS 数据分析中起着至关重要的作用，因为蛋白质形式的鉴定和表征通常需要手动检查数据，以确定高电荷离子的分子质量，并验证鉴定的蛋白质形式中是否存在意外的改变。虽然已经开发了许多用于 MS 数据可视化的软件工具，但仍然缺乏专门为自上而下的 MS 设计的基于网络的可视化软件。在这里，我们介绍了 TopMSV，这是一个用于自上而下的 MS 数据处理和可视化的基于网络的工具。TopMSV 使用网络浏览器提供自上而下的 MS 数据的交互式视图。它集成了用于光谱去卷积和蛋白质形式鉴定的软件工具，并使用工具的分析结果对自上而下的 MS 数据进行注释。
