Department of Emergency Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China.
Department of Gastroenterology, National Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China.
Microb Pathog. 2021 May;154:104849. doi: 10.1016/j.micpath.2021.104849. Epub 2021 Mar 27.
Acute pancreatitis (AP) has a broad spectrum of severity and is associated with considerable morbidity and mortality. Dysbiosis of gut microbiota may be associated with AP severity.
We aimed to evaluate the composition and functional effects of gut microbiota in different grades of AP severity.
We carried out shotgun metagenomic sequencing on rectal swab samples from three patients with mild acute pancreatitis (MAP), three with moderately severe acute pancreatitis (MSAP), three with severe acute pancreatitis (SAP) and three normal control persons (NOR). Differences analysis in gut microbiota composition and functional enrichment was performed.
Gut microbiota in AP patients was characterized by decreased species richness. The most representative gut microbiota in mild acute pancreatitis (MAP), moderately severe acute pancreatitis (MSAP), and severe acute pancreatitis (SAP) was Streptococcus, Escherichia-coli, and Enterococcus, respectively. Each of the three AP-associated genera could differentiate AP from healthy control population. Representative pathways associated with the glutathione metabolism, lipopolysaccharide biosynthesis, and amino acid metabolism (valine, leucine and isoleucine degradation) were enriched in MAP, MSAP, and SAP, respectively.
The study shows a potential association of gut microbiome composition and function to the progression of AP severity.
急性胰腺炎（AP）具有广泛的严重程度，并与相当大的发病率和死亡率相关。肠道微生物群落的失调可能与 AP 的严重程度有关。
我们旨在评估不同严重程度的 AP 患者肠道微生物群落的组成和功能效应。
我们对 3 例轻度急性胰腺炎（MAP）、3 例中度重症急性胰腺炎（MSAP）、3 例重症急性胰腺炎（SAP）和 3 例正常对照者（NOR）的直肠拭子样本进行了 shotgun 宏基因组测序。对肠道微生物群落组成和功能富集进行差异分析。
AP 患者的肠道微生物群落表现为物种丰富度降低。在轻度急性胰腺炎（MAP）、中度重症急性胰腺炎（MSAP）和重症急性胰腺炎（SAP）中，最具代表性的肠道微生物群落分别为链球菌、大肠杆菌和肠球菌。这三种与 AP 相关的属中的每一种都可以将 AP 与健康对照组区分开来。与谷胱甘肽代谢、脂多糖生物合成和氨基酸代谢（缬氨酸、亮氨酸和异亮氨酸降解）相关的代表性途径分别在 MAP、MSAP 和 SAP 中富集。
该研究表明肠道微生物群落组成和功能与 AP 严重程度的进展之间存在潜在的关联。
