Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Sci Rep. 2024 Sep 14;14(1):21491. doi: 10.1038/s41598-024-72057-z.
Acute pancreatitis (AP) is an inflammatory disease of the pancreas. Despite of a steadily increasing in morbidity and mortality, there is still no effective therapy. Gut microbial dysbiosis and its derived-metabolites disorder have been shown to play an important role in the development of AP, however, little is known regarding the crosstalk between gut microbiota and metabolites. In this study, we assessed the alterations in gut microbiota and metabolites by constructing three AP mouse models by means of metagenomic and metabolomic sequencing, and further clarified their relationship by correlation analysis. The results revealed that each model exhibited unique flora and metabolite profiles. KEGG analysis showed that the differential flora and metabolite-enriched pathway functions were correlated with lipid metabolism and amino acid metabolism. Moreover, two core differential bacterial species on Burkholderiales bacterium YL45 and Bifidobacterium pseudolongum along with eleven differential metabolites appeared to exert certain effects during the course of AP. In conclusion, further exploration of the crosstalk between microbiota and derived metabolites may provide novel insights and strategies into the diagnosis and treatment of AP.
急性胰腺炎（AP）是一种胰腺炎症性疾病。尽管发病率和死亡率稳步上升，但目前仍没有有效的治疗方法。肠道微生物失调及其衍生代谢物紊乱已被证明在 AP 的发展中起着重要作用，然而，关于肠道微生物群和代谢物之间的相互作用知之甚少。在这项研究中，我们通过构建三种 AP 小鼠模型，通过宏基因组和代谢组学测序来评估肠道微生物群和代谢物的变化，并通过相关分析进一步阐明它们之间的关系。结果表明，每种模型都表现出独特的菌群和代谢物特征。KEGG 分析表明，差异菌群和代谢物富集途径功能与脂质代谢和氨基酸代谢相关。此外，伯氏菌 YL45 和假长双歧杆菌这两种核心差异细菌物种以及十一种差异代谢物似乎在 AP 发病过程中发挥了一定的作用。总之，进一步探索微生物群和衍生代谢物之间的相互作用可能为 AP 的诊断和治疗提供新的见解和策略。
