College of Biological Science and Technology, Hubei Key Laboratory of Biological Resources Protection and Utilization, Key Laboratory of Green Manufacturing of Super-light Elastomer Materials of State Ethnic Affairs Commission, Hubei Minzu University, Enshi, China.
Turpan Vocational and Technical College, Turpan, China.
J Sci Food Agric. 2021 Oct;101(13):5660-5670. doi: 10.1002/jsfa.11220. Epub 2021 Apr 6.
Electrospun fibers are a good candidate for the delivery of bioactive compounds in the food industry because of their advantages that include a tunable diameter, high porosity and a high specific surface area. In the present study, we fabricated gelatin/glycerol monolaurate (GML) microemulsion nanofibers by solubilizing GML in Tween-80 followed by mixing with gelatin solution for electrospinning. We hypothesized that the addition of GML microemulsions affects the properties of the gelatin solution and modifies the physical and antimicrobial properties of the resulting nanofibers.
Both pure gelatin solution and gelatin/GML microemulsions showed shear-thinning behavior. However, electrospinnability was not affected by the addition of GML microemulsions. A significantly higher average diameter of nanofibers (1147 nm) with 5% GML was observed compared to the gelatin fiber diameter of 560 nm. Fourier transform infrared spectroscopy showed hydrogen bonding between gelatin molecules and GML microemulsions. Thermal analysis and X-ray diffraction indicated an amorphous structure of gelatin/GML microemulsion nanofibers, although a small amount of crystalline GML existed in the nanofibers with high GML content. Gelatin/GML microemulsion nanofibers showed high thermal stability and improved hydrophilicity. Nanofibers with 5% GML (weight with respect to nanofiber) (D64 nanofibers) showed effective antimicrobial activity against Escherichia coli and Staphylococcus aureus.
Gelatin/GML microemulsion nanofibrous films demonstrate superhydrophilicity and fast dissolution properties as a result of the high surface-to-volume ratio, amorphous structure and improved hydrophilicity of the nanofiber surface. The results indicate the potential application of gelatin/GML microemulsion nanofibrous films as edible antimicrobial food packaging. © 2021 Society of Chemical Industry.
电纺纤维因其具有可调节的直径、高孔隙率和高比表面积等优点，是在食品工业中输送生物活性化合物的良好候选材料。在本研究中，我们通过将 GML 溶解在 Tween-80 中，然后与明胶溶液混合进行静电纺丝，制备了明胶/甘油单月桂酸酯（GML）微乳液纳米纤维。我们假设添加 GML 微乳液会影响明胶溶液的性质，并改变所得纳米纤维的物理和抗菌性能。
纯明胶溶液和明胶/GML 微乳液均表现出剪切稀化行为。然而，添加 GML 微乳液并不影响电纺性能。与明胶纤维直径 560nm 相比，添加 5%GML 的明胶/GML 微乳液纳米纤维的平均直径（1147nm）显著增加。傅里叶变换红外光谱表明明胶分子与 GML 微乳液之间存在氢键。热分析和 X 射线衍射表明明胶/GML 微乳液纳米纤维为无定形结构，尽管在高 GML 含量的纳米纤维中存在少量结晶 GML。明胶/GML 微乳液纳米纤维具有较高的热稳定性和改善的亲水性。含有 5%GML（相对于纳米纤维的重量）（D64 纳米纤维）的纳米纤维对大肠杆菌和金黄色葡萄球菌表现出有效的抗菌活性。
由于高的比表面积、无定形结构和纳米纤维表面的改善的亲水性，明胶/GML 微乳液纳米纤维膜表现出超亲水性和快速溶解特性。结果表明，明胶/GML 微乳液纳米纤维膜作为可食用的抗菌食品包装具有潜在的应用前景。 © 2021 化学工业协会。
