Gao Linhui, Cai Peng, Zhou Yongjin J
Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Mar 25;37(3):966-979. doi: 10.13345/j.cjb.200645.
Methylotrophic yeasts are considered as promising cell factories for bio-manufacturing due to their several advantages such as tolerance to low pH and high temperature. In particular, their methanol utilization ability may help to establish a methanol biotransformation process, which will expand the substrate resource for bio-refinery and the product portfolio from methanol. This review summarize current progress on engineering methylotrophic yeasts for production of proteins and chemicals, and compare the strengths and weaknesses with the model yeast Saccharomyces cerevisiae. The challenges and possible solutions in metabolic engineering of methylotrophic yeasts are also discussed. With the developing efficient genetic tools and systems biology, the methylotrophic yeasts should play more important roles in future green bio-manufacturing.
甲基营养型酵母因其对低pH和高温的耐受性等多种优势，被视为生物制造领域颇具前景的细胞工厂。特别是，它们利用甲醇的能力有助于建立甲醇生物转化过程，这将扩大生物炼制的底物资源以及甲醇的产品组合。本综述总结了在工程改造甲基营养型酵母以生产蛋白质和化学品方面的当前进展，并与模式酵母酿酒酵母进行了优缺点比较。还讨论了甲基营养型酵母代谢工程中的挑战和可能的解决方案。随着高效遗传工具和系统生物学的发展，甲基营养型酵母在未来绿色生物制造中应发挥更重要的作用。
