Su Liqiu, Zhang Ge, Yao Zhen, Liang Peixin, Dai Zongjie, Wang Qinhong
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2021 May 25;37(5):1659-1676. doi: 10.13345/j.cjb.200707.
Over the past 30 years, Yarrowia lipolytica, Kluyveromyces, Pichia, Candida, Hansenula and other non-conventional yeasts have attracted wide attention because of their desirable phenotypes, such as rapid growth, capability of utilizing multiple substrates, and stress tolerance. A variety of synthetic biology tools are being developed for exploitation of their unique phenotypes, making them potential cell factories for the production of recombinant proteins and renewable bio-based chemicals. This review summarizes the gene editing tools and the metabolic engineering strategies recently developed for non-conventional yeasts. Moreover, the challenges and future perspectives for developing non-conventional yeasts into efficient cell factories for the production of useful products through metabolic engineering are discussed.
在过去30年里，解脂耶氏酵母、克鲁维酵母、毕赤酵母、假丝酵母、汉逊酵母等非常规酵母因其具有快速生长、能利用多种底物以及耐胁迫等理想表型而备受关注。人们正在开发各种合成生物学工具来利用它们的独特表型，使其成为生产重组蛋白和可再生生物基化学品的潜在细胞工厂。本文综述了最近为非常规酵母开发的基因编辑工具和代谢工程策略。此外，还讨论了通过代谢工程将非常规酵母发展成为生产有用产品的高效细胞工厂所面临的挑战和未来前景。
