LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465, Porto, Portugal.
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208, Matosinhos, Portugal.
FEMS Microbiol Ecol. 2021 Apr 13;97(5). doi: 10.1093/femsec/fiab052.
Although laboratory assays provide valuable information about the antifouling effectiveness of marine surfaces and the dynamics of biofilm formation, they may be laborious and time-consuming. This study aimed to determine the potential of short-time adhesion assays to estimate how biofilm development may proceed. The initial adhesion and cyanobacterial biofilm formation were evaluated using glass and polymer epoxy resin surfaces under different hydrodynamic conditions and were compared using linear regression models. For initial adhesion, the polymer epoxy resin surface was significantly associated with a lower number of adhered cells compared with glass (-1.27 × 105 cells.cm-2). Likewise, the number of adhered cells was significantly lower (-1.16 × 105 cells.cm-2) at 185 than at 40 rpm. This tendency was maintained during biofilm development and was supported by the biofilm wet weight, thickness, chlorophyll a content and structure. Results indicated a significant correlation between the number of adhered and biofilm cells (r = 0.800, p < 0.001). Moreover, the number of biofilm cells on day 42 was dependent on the number of adhered cells at the end of the initial adhesion and hydrodynamic conditions (R2 = 0.795, p < 0.001). These findings demonstrate the high potential of initial adhesion assays to estimate marine biofilm development.
虽然实验室检测可以提供有关海洋表面防污效果和生物膜形成动力学的有价值信息，但它们可能既繁琐又耗时。本研究旨在确定短期粘附检测的潜力，以估计生物膜的发展情况。使用玻璃和聚合物环氧树脂表面，在不同水动力条件下评估了初始粘附和蓝藻生物膜的形成，并使用线性回归模型进行了比较。对于初始粘附，与玻璃相比，聚合物环氧树脂表面与附着细胞数量较少显著相关（-1.27×105 个细胞/cm2）。同样，在 185 rpm 时附着的细胞数量也明显低于 40 rpm（-1.16×105 个细胞/cm2）。这种趋势在生物膜发育过程中得以维持，并得到生物膜湿重、厚度、叶绿素 a 含量和结构的支持。结果表明，附着细胞和生物膜细胞之间存在显著相关性（r=0.800，p<0.001）。此外，第 42 天生物膜细胞的数量取决于初始粘附结束时附着细胞的数量和水动力条件（R2=0.795，p<0.001）。这些发现表明，初始粘附检测具有很高的潜力，可以估计海洋生物膜的发展。
