Duan Ning, Guo Feng, Deng Bing, Yang Shaoxiang, Tian Hongyu, Sun Baoguo
Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing, China.
Luminescence. 2022 May;37(5):803-809. doi: 10.1002/bio.4224. Epub 2022 Mar 18.
A luminous intensity variation fluorescent probe (Probe 1) for the detection of ferric ion was developed. The quantitative range of Fe content detected was 0-600 μM with the limit of detection at 0.76 μM. Furthermore, after 20 min of Fe addition, the intensity of the luminescence of Probe 1 solution gradually decreased with increase in Fe concentration. In addition, the B and G values of these images showed a linear relationship with Fe concentration (0-500 μM). Probe 1 was successfully used for the rapid determination of Fe concentration in real samples. This study demonstrates that Probe 1 is an excellent tool for the rapid determination of Fe content in real samples using a smart phone without professional equipment.
开发了一种用于检测铁离子的发光强度变化荧光探针(探针1)。检测到的铁含量定量范围为0 - 600 μM,检测限为0.76 μM。此外,加入铁20分钟后,探针1溶液的发光强度随铁浓度的增加而逐渐降低。另外,这些图像的B值和G值与铁浓度(0 - 500 μM)呈线性关系。探针1成功用于实际样品中铁浓度的快速测定。本研究表明,探针1是一种无需专业设备即可使用智能手机快速测定实际样品中铁含量的优秀工具。