从人及小鼠细胞系、组织和体液中分离细胞外囊泡和颗粒。

Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids.

作者信息

Bojmar Linda, Kim Han Sang, Tobias Gabriel C, Pelissier Vatter Fanny A, Lucotti Serena, Gyan Kofi Ennu, Kenific Candia M, Wan Zurong, Kim Kyung-A, Kim DooA, Hernandez Jonathan, Pascual Virginia, Heaton Todd E, La Quaglia Michael P, Kelsen David, Trippett Tanya M, Jones David R, Jarnagin William R, Matei Irina R, Zhang Haiying, Hoshino Ayuko, Lyden David

机构信息

Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA.

Department of Biomedical and Clinical Sciences, Linköping University, Linköping 58183, Sweden.

出版信息

STAR Protoc. 2020 Dec 22;2(1):100225. doi: 10.1016/j.xpro.2020.100225. eCollection 2021 Mar 19.

We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).