Morey Amber, Ng Martin, Spanos Michail, Zhang Piyan, Xu Tuoye, Cheung Willi, Chatterjee Emeli, Gokulnath Priyanka, Carnel-Amar Natacha, Soares Chiaretti Ana Luisa, Nelson Collin, George Jubin, Luo Michelle, Chakraborty Abhik, Perucci Luiza, Jones Jennifer C, Hoff Peter De, Franklin Jeffrey L, Raffai Robert L, Das Saumya, Routenberg David A, Nolan John P, Charest Al, Laurent Louise C, Alexander Roger P
University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA.
Department of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, CA 94121, USA.
Extracell Vesicle. 2024 Jun;3. doi: 10.1016/j.vesic.2024.100040. Epub 2024 Apr 26.
Antibodies are critical tools for research into extracellular vesicles (EVs) and other extracellular nanoparticles (ENPs), where they can be used for their identification, characterization, and isolation. However, the lack of a centralized antibody platform where researchers can share validation results thus minimizing wasted personnel time and reagents, has been a significant obstacle. Moreover, because the performance of antibodies varies among assay types and conditions, detailed information on assay variables and protocols is also of value. To facilitate sharing of results on antibodies that are relevant to EV/ENP research, the EV Antibody Database has been developed by the investigators of the Extracellular RNA Communication Consortium (ERCC). Hosted by the ExRNA Portal (https://exrna.org/resources/evabdb/), this interactive database aggregates and shares results from antibodies that have been tested by research groups in the EV/ENP field. Currently, the EV Antibody Database includes modules for antibodies tested for western Blot, EV Flow Cytometry, and EV Sandwich Assays, and holds 110 records contributed by 6 laboratories from the ERCC. Detailed information on antibody sources, assay conditions, and results is provided, including negative results. We encourage ongoing expert input and community feedback to enhance the database's utility, making it a valuable resource for comprehensive validation data on antibodies and protocols in EV biology.
抗体是研究细胞外囊泡（EVs）和其他细胞外纳米颗粒（ENPs）的关键工具，可用于它们的鉴定、表征和分离。然而，缺乏一个研究人员可以共享验证结果从而最大限度减少人员时间和试剂浪费的集中式抗体平台，一直是一个重大障碍。此外，由于抗体的性能在不同检测类型和条件下有所不同，关于检测变量和方案的详细信息也很有价值。为了促进与EV/ENP研究相关抗体结果的共享，细胞外RNA通讯联盟（ERCC）的研究人员开发了EV抗体数据库。该交互式数据库由ExRNA门户网站（https://exrna.org/resources/evabdb/）托管，汇总并共享EV/ENP领域研究小组测试过的抗体结果。目前，EV抗体数据库包括用于蛋白质印迹、EV流式细胞术和EV夹心测定的抗体测试模块，并保存了来自ERCC的6个实验室提供的110条记录。提供了关于抗体来源、检测条件和结果的详细信息，包括阴性结果。我们鼓励持续的专家投入和社区反馈，以增强数据库的实用性，使其成为EV生物学中抗体和方案全面验证数据的宝贵资源。
