Pu Chun-Juan, Li Peng-Ying, Luo Yu-Zhi, Zhou Xiu-Teng, Shao Ai-Juan, Chen Mei-Lan
State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China China National Traditional Chinese Medicine Corporation Beijing 102600,China.
Zhongguo Zhong Yao Za Zhi. 2021 Mar;46(6):1368-1373. doi: 10.19540/j.cnki.cjcmm.20201222.101.
Arbuscular mycorrhizal fungi provided is beneficial to Salvia miltiorrhiza for increasing yield, promoting the accumulation of active ingredients, and alleviating S. miltiorrhiza disease etc. However, the application of fungicides will affect the benefit of arbuscular mycorrhizal fungi and there is little research about it. This article study the effect of four different fungicides: carbendazim, polyoxin, methyl mopazine, and mancozeb on mycorrhiza benefit to S. miltiorrhiza by the infection intensity of arbuscular mycorrhizal fungi, the growth of S. miltiorrhiza, and the content of active ingredients. RESULTS:: showed that different fungicides had different effects. The application of mancozeb had the strongest inhibitory effect on the mycorrhizal benefit to S. miltiorrhiza. Mancozeb significantly reduced the mycorrhizal colonization and the beneficial effect of arbuscular mycorrhizal fungi on the growth and the accumulation of active components of S. miltiorrhiza. The application of polyoxin had no significant effect on mycorrhizal colonization. Instead, it had a synergistic effect with the mycorrhizal benefit to promoting the growth and accumulation of rosmarinic acid of S. miltiorrhiza. The inhibitory strengths of four fungicides are: mancozeb>thiophanate methyl, carbendazim>polyoxin. Therefore, we recommend applying biological fungicides polyoxin and avoid applying chemical fungicides mancozeb for disease control during mycorrhizal cultivation of S. miltiorrhiza.
接种丛枝菌根真菌对丹参有益，可提高产量、促进活性成分积累并减轻丹参病害等。然而，杀菌剂的施用会影响丛枝菌根真菌的效益，对此相关研究较少。本文通过丛枝菌根真菌的侵染强度、丹参的生长情况以及活性成分含量，研究了四种不同杀菌剂（多菌灵、多抗霉素、甲基托布津和代森锰锌）对丛枝菌根真菌对丹参效益的影响。结果表明：不同杀菌剂的影响不同。代森锰锌对丛枝菌根真菌对丹参的效益抑制作用最强。代森锰锌显著降低了菌根定殖率以及丛枝菌根真菌对丹参生长和活性成分积累的有益作用。多抗霉素对菌根定殖无显著影响，反而与丛枝菌根真菌对促进丹参迷迭香酸生长和积累的效益具有协同作用。四种杀菌剂的抑制强度为：代森锰锌＞甲基托布津＞多菌灵＞多抗霉素。因此，我们建议在丹参菌根栽培过程中，为防治病害应施用生物杀菌剂多抗霉素，避免施用化学杀菌剂代森锰锌。
