Nanjing University of Chinese Medicine, Nanjing, 210023, China.
State Key Laboratory of Dao-Di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Sci Rep. 2022 Nov 16;12(1):19633. doi: 10.1038/s41598-022-17121-2.
Salvia miltiorrhiza Bunge (danshen in Chinese) is one of the most important medicinal cash crops in China. Previously, we showed that arbuscular mycorrhizal fungi (AMF) can promote S. miltiorrhiza growth and the accumulation of bioactive compounds. Fertilization may affect mycorrhizal efficiency, and appropriate doses of phosphate (P) and nitrogen (N) fertilizers are key factors for obtaining mycorrhizal benefits. However, the optimal fertilization amount for mycorrhizal S. miltiorrhiza remains unclear. In this study, we studied the effects of AMF on the growth and bioactive compounds of S. miltiorrhiza under different doses (low, medium, and high) of P and N fertilizer. The results showed that the mycorrhizal growth response (MGR) and mycorrhizal response of bioactive compounds (MBC) decreased gradually with increasing P addition. Application of a low (N25) dose of N fertilizer significantly increased the MGR of mycorrhizal S. miltiorrhiza, and a medium (N50) dose of N fertilizer significantly increased the MBC of phenolic acids, but decreased the MBC of tanshinones. Our results also showed that the existence of arbuscular mycorrhiza changes nutrient requirement pattern of S. miltiorrhiza. P is the limiting nutrient of non-mycorrhizal plants whereas N is the limiting nutrient of mycorrhizal plants.
丹参（Danshen in Chinese）是中国最重要的药用经济作物之一。此前，我们发现丛枝菌根真菌（AMF）可以促进丹参的生长和生物活性化合物的积累。施肥可能会影响菌根效率，而适当剂量的磷（P）和氮（N）肥是获得菌根效益的关键因素。然而，菌根丹参的最佳施肥量仍不清楚。在这项研究中，我们研究了不同剂量（低、中、高）的 P 和 N 肥下 AMF 对丹参生长和生物活性化合物的影响。结果表明，随着 P 添加量的增加，菌根生长响应（MGR）和生物活性化合物的菌根响应（MBC）逐渐降低。低（N25）剂量的 N 肥的应用显著增加了菌根丹参的 MGR，而中（N50）剂量的 N 肥显著增加了酚酸的 MBC，但降低了丹参酮的 MBC。我们的结果还表明，丛枝菌根的存在改变了丹参的营养需求模式。对于非菌根植物，P 是限制养分，而对于菌根植物，N 是限制养分。
