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一个含有整合 CC-BED 模块的长穗偃麦草 NLR 蛋白介导小麦白粉病抗性。

An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew.

机构信息

The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450046, P. R. China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

出版信息

Nat Commun. 2024 Sep 27;15(1):8281. doi: 10.1038/s41467-024-52670-2.

DOI:10.1038/s41467-024-52670-2
PMID:39333612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11436982/
Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), reduces wheat yields and grain quality, thus posing a significant threat to global food security. Wild relatives of wheat serve as valuable resources for resistance to powdery mildew. Here, the powdery mildew resistance gene Pm6Sl is cloned from the wild wheat species Aegilops longissima. It encodes a nucleotide-binding leucine-rich repeat (NLR) protein featuring a CC-BED module formed by a zinc finger BED (Znf-BED) domain integrated into the coiled-coil (CC) domain. The function of Pm6Sl is validated via mutagenesis, gene silencing, and transgenic assays. In addition, we develop a resistant germplasm harbouring Pm6Sl in a very small segment with no linkage drag along with the diagnostic gene marker pm6sl-1 to facilitate Pm6Sl deployment in wheat breeding programs. The cloning of Pm6Sl, a resistance gene with BED-NLR architecture, will increase our understanding of the molecular mechanisms underlying BED-NLR-mediated resistance to various pathogens.

摘要

白粉病由禾谷布氏白粉菌（Bgt）引起，降低了小麦的产量和品质，对全球粮食安全构成了重大威胁。小麦的野生近缘种是抗白粉病的宝贵资源。在这里，我们从野生小麦物种长穗偃麦草中克隆出了白粉病抗性基因 Pm6Sl。它编码一个核苷酸结合富含亮氨酸重复（NLR）蛋白，具有由锌指 BED（Znf-BED）结构域集成到卷曲螺旋（CC）结构域形成的 CC-BED 模块。通过突变、基因沉默和转基因试验验证了 Pm6Sl 的功能。此外，我们开发了一种携带 Pm6Sl 的抗性种质，该基因位于非常小的片段内，没有连锁拖曳，同时携带诊断基因标记 pm6sl-1，以促进 Pm6Sl 在小麦育种计划中的应用。BED-NLR 结构抗性基因 Pm6Sl 的克隆将提高我们对白粉病抗性的分子机制的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/a6824da99623/41467_2024_52670_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/3ba37b6b0bea/41467_2024_52670_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/ff5fe91b21d6/41467_2024_52670_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/17e0fb526f77/41467_2024_52670_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/107734d5724f/41467_2024_52670_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/39b5a2c16791/41467_2024_52670_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/a6824da99623/41467_2024_52670_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/3ba37b6b0bea/41467_2024_52670_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/ff5fe91b21d6/41467_2024_52670_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/17e0fb526f77/41467_2024_52670_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/107734d5724f/41467_2024_52670_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/39b5a2c16791/41467_2024_52670_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da88/11436982/a6824da99623/41467_2024_52670_Fig6_HTML.jpg

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Nat Commun. 2024 Apr 10;15(1):3124. doi: 10.1038/s41467-024-47497-w.
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Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein.
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