Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, P. R. China.
ACS Appl Mater Interfaces. 2021 Apr 14;13(14):16801-16811. doi: 10.1021/acsami.0c21109. Epub 2021 Mar 31.
The activity of a nanozyme is closely related to its surface area-to-volume ratio and the surrounding temperature. To acquire highly active nanozymes, one-pot metallization-like synthesis of novel nanoflower-shaped photothermal nanostructures was conducted using polyadenine-containing diblock DNA as the scaffold. The nanoflower-shaped structures with a high surface area-to-volume ratio and photothermal performance exhibited excellent peroxidase-mimicking activity, and the biorecognition capability was retained by the capping agent of diblock DNA. The functionalized nanostructures were used for a proof-of-concept colorimetric assay of cancer cells in vitro. Upon incorporation of 808 nm laser irradiation, high sensitivity and selectivity for the cancer cell assay were achieved with the lowest detection level of 10 cells/mL. Relative to spherical gold nanostructures, the nanoflower-shaped photothermal nanozyme exhibited higher assay sensitivity, paving the way for the construction of nanozyme-based colorimetric sensors for point-of-care testing.
纳米酶的活性与其表面积与体积比以及周围温度密切相关。为了获得高活性的纳米酶，采用含多聚腺嘌呤的嵌段 DNA 作为支架，通过类似一锅化金属的方法合成了新颖的纳米花状光热纳米结构。具有高表面积与体积比和光热性能的纳米花状结构表现出优异的过氧化物酶模拟活性，并且嵌段 DNA 的封端剂保留了其生物识别能力。该功能化的纳米结构被用于体外癌细胞的概念验证比色分析。通过掺入 808nm 激光照射，实现了对癌细胞分析的高灵敏度和选择性，最低检测水平达到 10 个细胞/mL。与球形金纳米结构相比，纳米花状光热纳米酶表现出更高的分析灵敏度，为基于纳米酶的即时检测比色传感器的构建铺平了道路。
