Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Arcavacata Rende (CS), Italy.
Department of Pharmacy, University of Salerno, Fisciano, Italy.
J Sci Food Agric. 2021 Nov;101(14):5872-5879. doi: 10.1002/jsfa.11239. Epub 2021 Apr 14.
This study investigated the chemical profile and biological activity of Diplotaxis erucoides subsp. erucoides (L.) DC. (Brassicaceae) collected in Sicily (Italy).
Liquid chromatography coupled with electrospray ionization and high-resolution mass spectrometry (LC-ESI/HRMS) analysis of the ethanol extract revealed the presence of 42 compounds - glucosinolates, hydroxycinnamic acids, flavonoids, and oxylipins. The extract was tested for its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), ferric reducing ability power (FRAP), and β-carotene bleaching tests. Promising protection from lipid peroxidation was observed after 30 min of incubation in a β-carotene bleaching test (IC of 3.32 μg mL ). The inhibition of carbohydrates-hydrolyzing enzymes resulted in IC values of 85.18 and 92.36 μg mL for α-amylase and α-glucosidase, respectively. Significant inhibition against lipase enzyme was observed (IC of 61.27 μg mL ).
Diplotaxis erucoides can be considered a potential source of antioxidant, hypoglycemic, and hypolipidemic bioactives. © 2021 Society of Chemical Industry.
本研究调查了西西里岛（意大利）采集的 Diplotaxis erucoides subsp. erucoides（十字花科）的化学成分和生物活性。
通过液相色谱-电喷雾电离-高分辨质谱联用（LC-ESI/HRMS）分析乙醇提取物，发现了 42 种化合物-硫代葡萄糖苷、羟基肉桂酸、类黄酮和氧化脂。采用 2,2-二苯基-1-苦基肼基（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）、铁还原能力（FRAP）和β-胡萝卜素漂白试验测定了提取物的抗氧化活性。在β-胡萝卜素漂白试验中孵育 30 分钟后观察到对脂质过氧化的显著保护作用（IC 为 3.32μgmL）。碳水化合物水解酶的抑制作用导致α-淀粉酶和α-葡萄糖苷酶的 IC 值分别为 85.18 和 92.36μgmL。对脂肪酶有显著抑制作用（IC 为 61.27μgmL）。
Diplotaxis erucoides 可被视为抗氧化、降血糖和降血脂生物活性的潜在来源。© 2021 化学工业协会。
