German Federal Institute for Risk Assessment, Department of Biological Safety, Berlin, Germany.
Der Grüne Zoo Wuppertal, Wuppertal, Germany.
J Clin Microbiol. 2021 May 19;59(6). doi: 10.1128/JCM.03125-20.
is an important pathogen for both humans and animals. It can infect livestock, as well as pets and wild animals. During recent years, a number of reports have described the isolation of from zoo animals, mainly birds and mammals, for which the infection was mostly lethal. Between 2005 and 2019, there were at least 17 cases of deceased mammals, belonging to five different species, which suffered from a infection at the Zoo Wuppertal, Germany. Since only scarce information exists on the properties of from zoo animals, we characterized eight isolates, covering all infected species, in detail. All isolates were members of biotype 1, but belonged to five serotypes, five sequence types (STs), and seven core-genome multilocus sequence types (cgMLSTs). Using pulsed-field gel electrophoresis (PFGE) analysis and whole-genome sequencing (WGS), the seven isolates could be discriminated from each other. They differed significantly regarding their virulence genes and mobile genetic elements. While the virulence plasmid pYV existed in all serotypes (five isolates), a complete high-pathogenicity island (HPI) was detected only in the serotypes O:1a, O:1b, and O:13 (four isolates), but not in O:2a and O:2b. Similarly, the content of other plasmids and prophages varied greatly between the isolates. The data demonstrate that the deceased mammals were infected by seven individual isolates and not by a single type predominating in the zoo animals.
是一种重要的人兽共患病病原体。它可以感染家畜、宠物和野生动物。近年来，有许多报道描述了从动物园动物（主要是鸟类和哺乳动物）中分离出的情况，这些感染大多是致命的。在 2005 年至 2019 年期间，德国伍珀塔尔动物园至少有 17 例不同物种的哺乳动物死亡，这些动物都感染了。由于来自动物园动物的的特性信息很少，我们详细表征了 8 个分离株，涵盖了所有感染的物种。所有分离株均属于生物型 1，但属于 5 个血清型、5 个序列型（ST）和 7 个核心基因组多位点序列型（cgMLST）。使用脉冲场凝胶电泳（PFGE）分析和全基因组测序（WGS），可以将这 7 个分离株彼此区分开来。它们在毒力基因和可移动遗传元件方面存在显著差异。虽然所有血清型（5 个分离株）都存在毒力质粒 pYV，但完整的高致病性岛（HPI）仅在血清型 O:1a、O:1b 和 O:13（4 个分离株）中检测到，而在 O:2a 和 O:2b 中则没有。同样，其他质粒和噬菌体的含量在分离株之间也有很大差异。这些数据表明，死亡的哺乳动物是由 7 个单独的分离株感染的，而不是由在动物园动物中占主导地位的单一类型感染的。
