快速耐碳青霉烯类鲍曼不动杆菌 NP 试验检测鲍曼不动杆菌的碳青霉烯类药物敏感性/耐药性。
Rapid ResaImipenem/Acinetobacter NP Test for Detection of Carbapenem Susceptibility/Resistance in Acinetobacter baumannii.
机构信息
Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland
INSERM European Unit (IAME), University of Fribourg, Fribourg, Switzerland.
出版信息
J Clin Microbiol. 2021 May 19;59(6). doi: 10.1128/JCM.03025-20.
The rapid ResaImipenem/ NP test was developed for the identification of carbapenem resistance among isolates. The principle of this test is based on the reduction of resazurin (a viability colorant) by metabolically active bacterial cells, hence detecting bacterial growth, in the presence of a defined concentration of imipenem chosen to be slightly above that defining imipenem resistance (6 μg/ml). Bacterial growth is visually detected by a color change from blue (resazurin) to purple or pink (resorufin product). A total of 110 isolates, among which 61 were imipenem resistant, were used to evaluate test performance. The sensitivity and specificity of the test were found to be 100%, in comparison with broth microdilution taken as the reference standard method. The rapid ResaImipenem/ NP test is highly specific and sensitive and is easy to implement in routine microbiology laboratories, and results are obtained within 2 h 30 min. It does not require any specific equipment.
快速亚胺培南/萘啶酮酸（ResaImipenem/NP）试验用于鉴定分离株中的碳青霉烯类耐药性。该试验的原理基于代谢活跃的细菌细胞还原亚甲蓝（一种生存力显色剂），从而在选择的稍高于定义碳青霉烯类耐药性的特定浓度亚胺培南（6μg/ml）存在下检测细菌生长。细菌生长通过颜色从蓝色（亚甲蓝）变为紫色或粉红色（产物 Resorufin）来肉眼检测。共有 110 株分离株，其中 61 株对亚胺培南耐药，用于评估试验性能。与作为参考标准方法的肉汤微量稀释法相比，该试验的灵敏度和特异性均为 100%。快速 ResaImipenem/NP 试验具有高度的特异性和敏感性，易于在常规微生物学实验室中实施，结果可在 2 小时 30 分钟内获得。它不需要任何特殊设备。
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