Sobin S S, Fung Y C, Tremer H M
Cardiovascular Research Laboratory, Los Angeles County-University of Southern California Medical Center 90033.
J Appl Physiol (1985). 1988 Apr;64(4):1659-75. doi: 10.1152/jappl.1988.64.4.1659.
The morphology and morphometric data of collagen and elastin fibers in the pulmonary alveolar walls are presented. Specimens were obtained from postmortem lungs quick-frozen at specified transpulmonary pressures. Collagen was stained by silver, and elastin was stained by orcein. Photomicrographs were composed by computer. Young lungs typically show small collagen fibers that radiate from the "posts," whereas larger fiber bundles traverse the septum irrespective of capillary blood vessels. In older lungs, rings of collagen around the posts appear enlarged. Elastin bundles do not show obvious variation in pattern with age and inflation pressure. Statistical frequency distributions of the fiber width and curvature are both skewed, but the square root of the width and the cube root of the curvature have approximate normal distributions. Typically, for young lungs at transpulmonary pressure of 4 cmH2O, the mean of (width)1/2 (in micron1/2) for collagen fibers is 0.952 +/- 0.242 (SD), that of (curvature)1/3 (in micron-1/3) is 0.349 +/- 0.094. The corresponding values for elastin are 0.986 +/- 0.255 and 0.395 +/- 0.094.
本文展示了肺泡壁中胶原纤维和弹性纤维的形态及形态测量数据。样本取自于在特定跨肺压下快速冷冻的尸检肺脏。胶原纤维用银染色，弹性纤维用orcein染色。显微照片由计算机合成。年轻的肺脏通常可见从“柱”处呈放射状的小胶原纤维，而较大的纤维束横穿间隔，与毛细血管无关。在老年肺脏中，“柱”周围的胶原环似乎增大。弹性纤维束的形态随年龄和充气压力未显示出明显变化。纤维宽度和曲率的统计频率分布均呈偏态，但宽度的平方根和曲率的立方根具有近似正态分布。通常，对于跨肺压为4 cmH2O的年轻肺脏，胶原纤维的（宽度）1/2（单位为微米1/2）平均值为0.952±0.242（标准差），（曲率）1/3（单位为微米-1/3）平均值为0.349±0.094。弹性纤维的相应值分别为0.986±0.255和0.395±0.094。
