Department of Biology, Institute of Natural and Applied Sciences, University of Van Yuzuncu Yil, Tuşba, Van, Turkey.
Department of Biology, Faculty of Science, University of Van Yuzuncu Yil, Tuşba, 65080, Van, Turkey.
Mol Biol Rep. 2021 Mar;48(3):2591-2599. doi: 10.1007/s11033-021-06310-3. Epub 2021 Mar 31.
Bisphenol F (BPF) has been used frequently in the plastics industry and the production of daily consumer products as an alternative to bisphenol A (BPA). It was aimed herein to determine the cytotoxic effects of BPF on hepatocytes isolated from the liver of rainbow trout (Oncorhyncus mykiss) using lactate dehydrogenase (LDH) assay and antioxidant defence system indicators. The cultured hepatocytes were exposed to seven concentrations (0, 15.63, 31.25, 62.50, 125, 250, and 500 µM) of BPF for 24 h. According to the LDH assay, the percentage of cytotoxicity was increased dose dependently in the cells. The malondialdehyde content, which is indicative of lipid peroxidation, was increased significantly at BPF concentrations between 15.63 and 250 µM, whereas it remained unchanged with a concentration of 500 µM. The activities of superoxide dismutase were increased, while those of catalase were decreased with all of the BPF concentrations. Elevated levels of reduced glutathione content were determined with BPF concentrations between 15.63 and 250 µM, but decreased significantly with a concentration of 500 µM. Significant increases in the activities of the glutathione peroxidase were found in hepatocytes treated with BPF at concentrations of 31.25 to 500 µM. GST activity was only significantly increased with a BPF concentration of 250 µM. The results showed that the toxic mechanism of BPF was mainly based on cell membrane damage and oxidative stress, which have an influence on antioxidant defences. Therefore, BPF should be reconsidered as a safe alternative instead of BPA in the manufacturing of industrial or daily products.
双酚 F（BPF）在塑料工业和日常消费品生产中被频繁用作双酚 A（BPA）的替代品。本研究旨在通过乳酸脱氢酶（LDH）测定法和抗氧化防御系统指标，确定 BPF 对虹鳟（Oncorhyncus mykiss）肝脏分离肝细胞的细胞毒性作用。将培养的肝细胞暴露于七种浓度（0、15.63、31.25、62.50、125、250 和 500 μM）的 BPF 中 24 小时。根据 LDH 测定法，细胞的细胞毒性呈剂量依赖性增加。丙二醛含量（指示脂质过氧化）在 15.63 至 250 μM 的 BPF 浓度下显著增加，而在 500 μM 的浓度下保持不变。超氧化物歧化酶的活性增加，而过氧化氢酶的活性则降低，所有 BPF 浓度下均如此。谷胱甘肽还原含量随着 BPF 浓度在 15.63 至 250 μM 之间的增加而升高，但在 500 μM 的浓度下显著降低。用 31.25 至 500 μM 的 BPF 处理肝细胞，发现谷胱甘肽过氧化物酶的活性显著增加。只有在 BPF 浓度为 250 μM 时，GST 活性才会显著增加。结果表明，BPF 的毒性机制主要基于细胞膜损伤和氧化应激，这对抗氧化防御有影响。因此，在制造工业或日常产品时，BPF 应重新被视为 BPA 的安全替代品。
