Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo, 11566, Egypt.
Mol Biol Rep. 2021 Mar;48(3):2619-2628. doi: 10.1007/s11033-021-06316-x. Epub 2021 Apr 1.
Fabaceae, the third-largest Angiosperm family, exhibits great morphological diversity with significantly high species diversification rate. Albizia, one of the largest genera of the legume family, possesses high ecological, economical and medicinal application prospects and displays a global distribution. The taxonomic classification among Albizia remains, however, unclear and has been subjected to changes. The resolution of phylogenetic relationships among members of genus Albizia is a priority. Nine Albizia species cultivated in Egypt; Albizia lebbeck, A. julibrissin, A. odoratissima, A. procera, A. anthelmintica, A. guachapele, A. myriophylla, A. richardiana and A. lucida were subjected to molecular classification via DNA fingerprinting techniques viz. Inter Simple Sequence Repeat (ISSR) and Start Codon Targeted polymorphism (SCoT) using ten primers, five for each technique. The total number of bands produced by ISSR and SCoT primers was 28 and 40, respectively. The percentage of polymorphism varied from 64.28% in ISSR to 67.50% in SCoT analysis. Additionally, chemotaxonomic analysis was implemented based on UV spectroscopic profiling and total phenolic content coupled to unsupervised chemometric tools; Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA). Interspecific relationships were confirmed via molecular and phytochemical analyses between A. procera and A. guachapele; A. lebbeck and A. odoratissima; and A. julibrissin and A. lucida. The study reveals that chemotaxonomic data can reflect phylogenetic relationships among examined Albizia species and provides insights to the significance of utilizing the strengths of both molecular taxonomy and chemotaxonomy to resolve phylogenetic relationship among this genus which offers baseline for breeding programs. Future strategies to enrich taxonomic classification among Albizia includes extensive morphological characterization, DNA barcoding techniques and metabolomic profiling.
豆科是被子植物第三大科，具有显著的形态多样性和较高的物种分化速率。其中，合欢属是豆科最大的属之一，具有很高的生态、经济和药用应用前景，并且分布广泛。然而，合欢属的分类仍然不清楚，并且一直在变化。解决合欢属成员之间的系统发育关系是当务之急。对埃及栽培的 9 种合欢属植物（A. lebbeck、A. julibrissin、A. odoratissima、A. procera、A. anthelmintica、A. guachapele、A. myriophylla、A. richardiana 和 A. lucida）进行了分子分类，使用 DNA 指纹图谱技术（ISSR 和 SCoT），用 10 个引物，每个技术 5 个引物。ISSR 和 SCoT 引物产生的总条带数分别为 28 和 40，多态性百分比分别为 ISSR 中的 64.28%和 SCoT 分析中的 67.50%。此外，还进行了化学分类学分析，基于紫外光谱分析和总酚含量，并结合无监督化学计量工具；主成分分析（PCA）和层次聚类分析（HCA）。通过分子和植物化学分析证实了 A. procera 和 A. guachapele；A. lebbeck 和 A. odoratissima；以及 A. julibrissin 和 A. lucida 之间的种间关系。研究表明，化学分类学数据可以反映所研究的合欢属物种之间的系统发育关系，并为利用分子分类学和化学分类学的优势来解决该属的系统发育关系提供了见解，这为育种计划提供了基础。今后丰富合欢属分类的策略包括广泛的形态特征描述、DNA 条形码技术和代谢组学分析。
