Li Xinpei, Zhou Qianjie, Ma Litao, Chen Kun, Yin Panchao
South China Advanced Institute for Soft Matter Science and Technology & State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, PR China.
South China Advanced Institute for Soft Matter Science and Technology & State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, PR China.
J Colloid Interface Sci. 2021 Jul 15;594:874-881. doi: 10.1016/j.jcis.2021.03.090. Epub 2021 Mar 19.
The hybridization of enzymes and inorganics in controlled manner is challenging, however, critical for the development of chemo-enzymatic cascade catalyst with high efficiency and selectivity. Here, proteins and metal oxide clusters can be facilely co-assembled on the surface of colloid of poly(4-vinylpyridine) (P4VP) via hydrogen bonding, due to their enriched surface hydrogen bonding donors. The co-assembly method can be generally applied for preparing chemo-enzymatic catalyst within the selected database of various proteins and metal oxide clusters while the assembly units retain their structures and activities. Typically, a 2.5 nm metal oxide cluster {MoFe}, with peroxidase-like activity, are complexed with glucose oxidase (GOX) on P4VP for the catalysis against the oxidization of o-dianisidine (ODA) with the existence of glucose. Due to the synergistic effects of chemical and enzymatic catalysis, the co-assemblies show even higher ODA oxidation activity compared to GOX/catalase bi-enzymatic system, confirming the effectiveness of the co-assembly protocol for cascade catalysis and enabling its applications in rapid glucose detection and biomass conversion.
以可控方式实现酶与无机物的杂化具有挑战性，然而，这对于高效且选择性高的化学酶级联催化剂的开发至关重要。在此，由于蛋白质和金属氧化物簇富含表面氢键供体，它们可通过氢键在聚（4-乙烯基吡啶）（P4VP）胶体表面轻松共组装。该共组装方法通常可用于在各种蛋白质和金属氧化物簇的选定数据库中制备化学酶催化剂，同时组装单元保留其结构和活性。典型地，具有类过氧化物酶活性的2.5纳米金属氧化物簇{MoFe}与葡萄糖氧化酶（GOX）在P4VP上络合，用于在葡萄糖存在下催化邻联茴香胺（ODA）的氧化。由于化学催化和酶催化的协同作用，与GOX/过氧化氢酶双酶体系相比，共组装体表现出更高的ODA氧化活性，证实了共组装方案用于级联催化的有效性，并使其能够应用于快速葡萄糖检测和生物质转化。
