Brown C S, Van Lent J W, Vlak J M, Spaan W J
Department of Virology, Faculty of Medicine, Leiden University, The Netherlands.
J Virol. 1991 May;65(5):2702-6. doi: 10.1128/JVI.65.5.2702-2706.1991.
Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.
空的细小病毒B19衣壳是从感染了仅表达细小病毒B19 VP2的重组杆状病毒以及同时表达VP1和VP2的双重组杆状病毒的昆虫细胞中分离出来的。仅VP2就能组装形成衣壳，这是细小病毒中以前未观察到的现象。同时含有VP1和VP2的衣壳的化学计量与以前在感染细小病毒B19的细胞中观察到的相似。这些衣壳在大小和外观上与天然衣壳相似，并且通过用B19特异性抗体进行免疫沉淀和酶联免疫吸附测定证明了它们的抗原性。
