Nigoghosian Gregory, Weidner Lauren M, Stamper Trevor I
Department of Entomology, Purdue University, 901 W. State Street, West Lafayette, IN, 47907, USA.
Forensic Sci Int Synerg. 2021 Jan 22;3:100135. doi: 10.1016/j.fsisyn.2021.100135. eCollection 2021.
Geometric morphometric analyses use landmark coordinates from images of specific areas on specimens. Therefore, to minimize differences arising from human preparation, preparation of the specimen is crucial. The application of geometric morphometrics has been used in entomology for identifying adult dipteran species, however, applying this technique to larval specimens requires the production of standard protocols which would allow specimens to be compared equally. This proposed protocol consists of a two-step process: 1. using 15% potassium hydroxide (KOH) to clear the specimen, followed by rinsing the specimen with 80% ethanol; 2. removal and spreading of the mouthhooks on a slide for imaging and analysis. The goal of this work is to standardize specimen preparation, which would reduce human error and facilitate use of geometric morphometrics to identify dipteran larvae.
几何形态测量分析使用标本特定区域图像中的地标坐标。因此，为了尽量减少人为制备过程中产生的差异，标本的制备至关重要。几何形态测量学已应用于昆虫学领域以识别成年双翅目物种，然而，将该技术应用于幼虫标本需要制定标准方案，以便能够对标本进行平等比较。本提议的方案包括两个步骤：1. 使用15%的氢氧化钾（KOH）清理标本，然后用80%的乙醇冲洗标本；2. 将口钩取下并铺展在载玻片上以便成像和分析。这项工作的目标是使标本制备标准化，这将减少人为误差，并便于使用几何形态测量学来识别双翅目幼虫。
