Department of Urology, School of Medicine, Dokuz Eylul University, Izmir, Turkey.
Department of Urology, Mus Malazgirt State Hospital, Mus, Turkey.
Int J Clin Pract. 2021 Jul;75(7):e14193. doi: 10.1111/ijcp.14193. Epub 2021 Apr 12.
The aim of this study is to establish the optimal non-invasive urine sample collection method for the microbiota studies.
Twelve men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analysed using V3-V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analysed in first voided urine and midstream urine samples at phylum, class, order and family level. At the genus level, all of the identified bacterial groups' relative abundances were analysed. The statistical significance (P < .05) of differences between first voided and midstream urine sample microbiota was evaluated using the Wilcoxon test.
According to the analysis, 8 phyla, 14 class, 23 orders, 39 families and 29 different genera were identified in the first voided and the midstream urine samples. Statistical differences were not identified between first voided and midstream urine samples of all bacteria groups except the Clostridiales at order level (p:0.04) and Clostridia at class level (P: .04).
Either first voided or midstream urine samples can be used in urinary microbiota studies as we determined that there is no statistically significant difference between them regarding the results of 16s ribosomal RNA analysis.
本研究旨在确定用于微生物组研究的最佳非侵入性尿液样本采集方法。
12 名膀胱癌男性患者进行首次排空和中段尿液采集。使用细菌 16s 核糖体 RNA 的 V3-V4 区分析尿液样本。在门、纲、目和科水平上分析相对丰度大于 1%的细菌群在首次排空尿液和中段尿液样本中的分布。在属水平上，分析所有鉴定出的细菌群的相对丰度。使用 Wilcoxon 检验评估首次排空尿液和中段尿液样本微生物组之间的统计学差异（P<.05）。
根据分析，在首次排空和中段尿液样本中鉴定出 8 个门、14 个纲、23 个目、39 个科和 29 个不同的属。除了在目水平上的梭菌目（P：0.04）和纲水平上的梭菌纲（P：0.04）的所有细菌群外，首次排空和中段尿液样本之间未发现统计学差异。
无论首次排空尿液还是中段尿液样本都可用于尿液微生物组研究，因为我们确定在 16s 核糖体 RNA 分析结果方面，两者之间没有统计学上的显著差异。
