Li Zhilei, Zhou Jianping, Dong Tao, Xu Yan, Shang Yukui
College of Mechanical Engineering, Xinjiang University, Urumchi, 830047, China; Engineering Training Center of Xinjiang University, Urumchi, 830047, China.
College of Mechanical Engineering, Xinjiang University, Urumchi, 830047, China.
Biosens Bioelectron. 2021 Jun 15;182:113105. doi: 10.1016/j.bios.2021.113105. Epub 2021 Mar 9.
Abiotic stress is the main cause of low productivity in plants. Therefore, it is important to detect stress and respond to it in a timely manner to avoid irreversible damage to plant productivity and health. The application of traditional methods in agriculture is limited by expensive equipment and cumbersome sample processing. More effective detection methods are urgently needed due to the trace amounts and low stabilities of plant biomarkers. Electrochemical detection methods have the unique advantages of high accuracy, a low detection limit, fast response and easy integration with systems. In this review, the application of three types of electrochemical methods to phytohormone assessment is highlighted including direct electrochemical, immunoelectrochemical, and photoelectrochemical methods. Research on electrochemical methods for detecting abiotic stress biomarkers, including various phytohormones, is also summarized with examples. To date, the detection limit of exogenous plant hormones can reach pg/mL or even lower. Nevertheless, more efforts need to be made to develop a portable instrument for in situ online detection if electrochemical sensors are to be applied to the detection of the endogenous hormones or the physiological state of plants. Additionally, plant-wearable sensors that can be directly attached to or implanted into plants for continuous, noninvasive and real-time monitoring are emphasized. Finally, rational summaries of the considered methods and present challenges and future prospects in the field of abiotic stress detection-based electrochemical biosensors are thoroughly discussed.
非生物胁迫是植物生产力低下的主要原因。因此，及时检测胁迫并做出响应以避免对植物生产力和健康造成不可逆转的损害至关重要。传统方法在农业中的应用受到昂贵设备和繁琐样品处理的限制。由于植物生物标志物的痕量和低稳定性，迫切需要更有效的检测方法。电化学检测方法具有高精度、低检测限、快速响应以及易于与系统集成的独特优势。在本综述中，重点介绍了三种电化学方法在植物激素评估中的应用，包括直接电化学法、免疫电化学法和光电化学法。还举例总结了检测非生物胁迫生物标志物（包括各种植物激素）的电化学方法的研究。迄今为止，外源植物激素的检测限可达到pg/mL甚至更低。然而，如果要将电化学传感器应用于内源激素或植物生理状态的检测，还需要做出更多努力来开发用于原位在线检测的便携式仪器。此外，强调了可直接附着于植物或植入植物以进行连续、无创和实时监测的植物可穿戴传感器。最后，对所考虑的方法进行了合理总结，并深入讨论了基于非生物胁迫检测的电化学生物传感器领域当前的挑战和未来前景。
