Velásquez-Orozco Fernando, Rando-Segura Ariadna, Martínez-Camprecios Joan, Salmeron Paula, Najarro-Centeno Adrián, Esteban Àngels, Quer Josep, Buti María, Pumarola-Suñe Tomás, Rodríguez-Frías Francisco
Department of Microbiology, Vall d'Hebron Hospital Universitari, Vall d'Hebron Barcelona Hospital Campus, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain.
Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Diagnostics (Basel). 2021 Mar 8;11(3):473. doi: 10.3390/diagnostics11030473.
Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests. The aim of this study was to compare the performance of dried plasma spots (DPS), prepared using the cobas Plasma Separation Card (PSC), to plasma and serum from venipuncture, for HCV diagnosis. We carried out a prospective study using DPS and paired plasma or serum samples. Serum and DPS samples were analyzed by immunoassay using Elecsys Anti-HCV II (Roche). Plasma and DPS samples were analyzed using the cobas HCV viral load and cobas HCV genotyping tests (Roche). All DPS samples that had high anti-HCV antibody titers in serum were also antibody-positive, as were five of eight samples with moderate titers. Eight samples with low titers in serum were negative with DPS. Among 80 samples with plasma HCV viral loads between 61.5 and 2.2 × 10 IU/mL, 74 were RNA-positive in DPS. The mean viral load difference between plasma and DPS was 2.65 log IU/mL. The performance of DPS for detection of serological and virological markers of hepatitis C virus infection was comparable to that of the conventional specimen types. However, the limits of detection were higher for DPS.
丙型肝炎病毒（HCV）感染者的诊断和临床管理依赖于血清学和病毒学检测结果的综合。本研究的目的是比较使用 cobas 血浆分离卡（PSC）制备的干血浆斑（DPS）与静脉穿刺采集的血浆和血清在 HCV 诊断方面的性能。我们使用 DPS 和配对的血浆或血清样本进行了一项前瞻性研究。血清和 DPS 样本采用 Elecsys Anti-HCV II（罗氏）免疫测定法进行分析。血浆和 DPS 样本使用 cobas HCV 病毒载量检测和 cobas HCV 基因分型检测（罗氏）进行分析。血清中抗 HCV 抗体滴度高的所有 DPS 样本抗体也呈阳性，血清滴度中等的 8 个样本中有 5 个也是如此。血清滴度低的 8 个样本 DPS 检测为阴性。在 80 个血浆 HCV 病毒载量在 61.5 至 2.2×10 IU/mL 之间的样本中，74 个在 DPS 中 RNA 呈阳性。血浆和 DPS 之间的平均病毒载量差异为 2.65 log IU/mL。DPS 在检测丙型肝炎病毒感染的血清学和病毒学标志物方面的性能与传统样本类型相当。然而，DPS 的检测限更高。
