Alotaibi Khalid M, Almethen Abdurrahman A, Beagan Abeer M, Alfhaid Latifah H, Ahamed Maqusood, El-Toni Ahmed M, Alswieleh Abdullah M
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.
Polymers (Basel). 2021 Mar 8;13(5):823. doi: 10.3390/polym13050823.
In this paper, a new pH-responsive nanosystem based on mesoporous silica nanoparticles (MSNs) was developed for cancer therapy. Poly(2-(diethylamino) ethyl methacrylate) (PDEAEMA) was grafted on their outer surface and acts as a gatekeeper, followed by subsequent modification of the polymer by cysteine (MSN-PDEAEMA-Cys) and poly(oligo(ethylene glycol) methyl ether methacrylate) (MSN-PDEAEMA-Cys-POEGMEMA). The physicochemical properties of these nanocarriers were characterized using scanning and transmission electron microscopies (SEM and TEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS). The synthesized nanoparticles were well-dispersed with a diameter of ca. 200 nm. The obtained XPS results confirm the successful modification of MSN-PDEAEMA with Cys and POEGMEMA by increasing the peak intensity of C-O and C=O groups at 286.5 and 288.5 eV, respectively. An anti-cancer drug, doxorubicin (DOX), was encapsulated into the fabricated nanoplatform. The DOX release amount at physiological pH of 7.4 was limited (10%), while an accumulation drug release of ca. 35% was accomplished after 30 h in acidic media. The MTT cell line was used to assess the cytotoxicity of the unloaded and DOX-loaded fabricated nanoplatforms. Upon loading of DOX on these nanomaterials, they showed significant toxicity to human liver cancer cells. These results suggest that the prepared nano-structured materials showed good biocompatibility as well, and they can serve as nanocarriers for the delivery of anti-cancer drugs.
在本文中，开发了一种基于介孔二氧化硅纳米颗粒（MSN）的新型pH响应纳米系统用于癌症治疗。聚（甲基丙烯酸2-（二乙氨基）乙酯）（PDEAEMA）接枝在其外表面并充当守门人，随后通过半胱氨酸（MSN-PDEAEMA-Cys）和聚（寡聚（乙二醇）甲基醚甲基丙烯酸酯）（MSN-PDEAEMA-Cys-POEGMEMA）对聚合物进行后续修饰。使用扫描和透射电子显微镜（SEM和TEM）、傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和动态光散射（DLS）对这些纳米载体的物理化学性质进行了表征。合成的纳米颗粒分散良好，直径约为200 nm。获得的XPS结果证实了通过分别增加286.5和288.5 eV处C-O和C=O基团的峰强度，成功地用半胱氨酸和POEGMEMA对MSN-PDEAEMA进行了修饰。将一种抗癌药物阿霉素（DOX）封装到制备的纳米平台中。在生理pH值7.4时，DOX的释放量有限（10%），而在酸性介质中30小时后实现了约35%的累积药物释放。使用MTT细胞系评估未负载和负载DOX的制备纳米平台的细胞毒性。在这些纳米材料上负载DOX后，它们对人肝癌细胞显示出显著的毒性。这些结果表明，制备的纳米结构材料也显示出良好的生物相容性，并且它们可以作为抗癌药物递送的纳米载体。
