TransMIT Center for Effect-Directed Analysis, and Chair of Food Science, Institute of Nutritional Science, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
PiLeJe Industrie, Naturopôle Nutrition Santé, Les Tiolans, 03800 Saint-Bonnet-de-Rochefort, France.
Molecules. 2021 Mar 8;26(5):1468. doi: 10.3390/molecules26051468.
An effect-directed profiling method was developed to investigate 17 different fortified plant extracts for potential benefits. Six planar effect-directed assays were piezoelectrically sprayed on the samples separated side-by-side by high-performance thin-layer chromatography. Multipotent compounds with antibacterial, α-glucosidase, β-glucosidase, AChE, tyrosinase and/or β-glucuronidase-inhibiting effects were detected in most fortified plant extracts. A comparatively high level of antimicrobial activity was observed for , hops, grape pomace, passiflora, rosemary and . Except in red vine, black radish and horse tail, strong enzyme inhibiting compounds were also detected. Most plants with anti-α-glucosidase activity also inhibited β-glucosidase. Green tea, lemon balm and rosemary were identified as multipotent plants. Their multipotent compound zones were characterized by high-resolution mass spectrometry to be catechins, rosmarinic acid, chlorogenic acid and gallic acid. The results pointed to antibacterial and enzymatic effects that were not yet known for plants such as and for compounds such as cynaratriol and caffeine. The nontarget effect-directed profiling with multi-imaging is of high benefit for routine inspections, as it provides comprehensive information on the quality and safety of the plant extracts with respect to the global production chain. In this study, it not only confirmed what was expected, but also identified multipotent plants and compounds, and revealed new bioactivity effects.
建立了一种定向作用分析方法，以研究 17 种不同强化植物提取物的潜在益处。通过压电喷雾，将六种平面定向作用分析方法分别喷涂在高效薄层色谱分离的样品上。在大多数强化植物提取物中，检测到具有抗菌、α-葡萄糖苷酶、β-葡萄糖苷酶、AChE、酪氨酸酶和/或β-葡萄糖醛酸酶抑制作用的多功能化合物。、啤酒花、葡萄渣、西番莲、迷迭香和在大多数强化植物提取物中，检测到具有抗菌、α-葡萄糖苷酶、β-葡萄糖苷酶、AChE、酪氨酸酶和/或β-葡萄糖醛酸酶抑制作用的多功能化合物。除了红葡萄和黑萝卜和马尾草，还检测到了具有强烈酶抑制作用的化合物。大多数具有抗α-葡萄糖苷酶活性的植物也抑制β-葡萄糖苷酶。绿茶、柠檬香脂和迷迭香被鉴定为多能植物。它们的多能化合物区域通过高分辨率质谱法被鉴定为儿茶素、迷迭香酸、绿原酸和没食子酸。结果表明，一些植物如和一些化合物如 Cynaratriol 和咖啡因具有抗菌和酶抑制作用，而这些作用在以前并不为人所知。多成像的非靶向作用分析对常规检查具有很高的益处，因为它提供了有关植物提取物的质量和安全性的全面信息，涉及全球生产链。在这项研究中，它不仅证实了预期的结果，而且还鉴定了多能植物和化合物，并揭示了新的生物活性作用。
